... site-directed mutagenesis; squalene cyclase; thiol
reagents.
Oxidosqualene cyclases (OSCs) and squalene- hopene
cyclases (SHCs) are key enzymes in triterpenoid biosynthesis:
they transform acyclic isoprenoid ... Novara, Italy
The function of squalene- hopene cyclase from Alicycloba-
cillus acidocaldarius was studied by labelling critical cysteine
residues of the enzyme, either native...
... analysis of sol-
id-pseudopapillary tumor of the pancreas: report of 15 cases.
Hepatobilliary Pancreat Dis Int. 2008;7(2):196-200.
10. Yu CC, Yeh CN, Hwang TL, Jan YY. Clinicopathological study ... routinely
performed to rule out pancreatic malignancy. Other-
wise, for the patients with pancreatic malignancies, a
radical surgery should be the reasonable option for
curative therapy. A...
... laboratories, which were performing
confirmatory testing for HB in each jurisdiction, either
automatically reported or were contacted regularly for
newly identified HB cases, laboratory test results, ... predefined questionnaires for all
seven health regions. The notification system was not
materially changed during the study period, but
augmented throughout by scrutiny of laborat...
... 1,6-bisphosphatase
aldolase
4 GlyA Serine hydroxymethyltransferase
5 LpdA* (AtpA) Lipoamide dehydrogenase
(F1 ATP synthase a subunit)
6 Ppa* (AhpC) Inorganic pyrophosphatase
(alkyl hydroperoxide reductase ... BL21.
For purification of zinc-binding proteins, cells were lysed
in lysis buffer containing 0.2 mgÆmL
)1
lysozyme, 0.2 m
M
phenylmethanesulfonyl fluoride and 1 m
M
dithiothreitol,
follo...
... namely that they contain a
hydrogen-bond donor in wing2. This property is of general
importance for the efficacy of the inhibitor. Substitution of
the hydrogen-donating amide group for a methylene ... The analysis of the inhibitors HBY 097 in complex
with the Y1 88L mutant and 8-Cl TIBO in complex with the
Y1 81C mutant, revealed that the retained efficacy of these
inhibitors was due...
... the mouse aryl hydrocarbon receptor (mAhR).
The model allowed us to putatively i dentify the residues
responsible for the recognition of polychlorinated dibenzo-
p-dioxins (PCDDs) by AhR receptors ... FixL, oxygen binding at the heme
binding PAS domain controls the activity of a h istidine
kinase domain [12]; and in PYP, a local conformational
change occurs once the p-hydroxycinnamoyl cromo...
... algorithm for computing efficiently the expected
counts of any sequence in a word lattice output by a
speech recognizer or any arbitrary weighted automaton;
describe a new technique for creating ... weight function;
and the final weight function mapping to
.
A Weighted automaton is de-
fined in a similar way by simply omitting the output la-
bels. We denote by the set of strings accepted...
... for Field Work in Phonology
by Joseph E. Grimes, John R. Alsop, and Alan Wares, Summer Institute of Linguistics
In the study of a previously unrecorded language, a taxonomy of the
sound system ... phonological taxonomy is no longer by
itself a final goal in linguistic analysis, a linguist who tries
to study the phonology of a language without first mak-
ing a good taxonomy stands as m...
... relatively small in the dialogue
management task, our state space is determined by
the underlying environment and is typically quite
large. We address this complexity by developing
a policy gradient ... user.
Reinforcement learning is applied very differ-
ently in dialogue systems compared to our setup.
In some respects, our task is more easily amenable
to reinforcement learning. For instan...
...
While
this form of attachment ambiguity is usually
easy for people to resolve, a computer requires
detailed knowledge about words (e.g.,
washed
vs.
bought)
in order to successfully resolve ... the
form
(v, n,p,
n2), in which
both
the noun n and
verb v are always known. We therefore must
use any information in the unambiguous cases
to resolve the ambiguous cases. A natural wa...