Báo cáo Y học: The role of zinc in the methylation of the coenzyme M thiol group in methanol:coenzyme M methyltransferase from Methanosarcina barkeri New insights from X-ray absorption spectroscopy doc
Báo cáo y học: "Why are some children with early onset of asthma getting better over the years" -aneuploid-prostate-cancer-cells-after-tmz-tmz-bioshuttle-treatment.html#post143974
... University Hospital, including PHC data of the statements made by the mid-wife in her records of the check-up of the mothers during the pregnancy (17). In the follow-up at the age of seven, ... asthma remain asymptomatic without needing ther-apy for the rest of their lives, but asthma remission also does exist (12). The more severe the asthma is in childhood the more likely it is ... also indicated. A renewed analysis of the medical records in the NA group by one of us (E.R.) fully confirmed the ini-tial asthma diagnosis in 44.4 %, partially in 42.9 %, whereas in 12.7% other...
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Báo cáo y học: "Grb2-associated binder 1 polymorphism was associated with the risk of Helicobactor pylori infection and gastric atrophy"
... The Gab1 polymorphism may affect the interaction with SHP-2 through the mechanism such as BTAM after H pylori infection, resulting in influencing the abnormal proliferation and movement of ... domains or by specific phosphopeptides that bind to the SH2 domains. The tandem SH2 domains bind to Tyr-627 and Tyr-659 simultaneously in a specific orientation, in which Tyr-627 binds to the ... obtained from all the subjects. This study protocol was approved by the Ethics Committee of the Nagoya University Graduate School of Medicine. Tests for Helicobacer pylori (H. pylori) antibody...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... the activity of the enzyme was measured by the dinitrosalicylicacid method.Inhibition of the enzyme activity by glucose, maltose andcyclohexaamyloseFor the study of the enzyme inhibition by glucose, ... enzymewas determined using the Betamyl b-amylase test reagent(Megazyme, Wicklow, Ireland).To determine the pH stability of the b-amylase, aliquots of a solution of the purified protein (4.06 mg·mL21 in ... of the b-amylase from hedge bindweed and the model b-amylase from soybean. HCA plots were generated using the programHCA-Plot2 (Doriane, Paris, France).Molecular modeling of the b-amylase from...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc
... between the buffer and the enzyme by preincubating the enzyme–metal mixture at30 8C for 30 min. Remaining activity was measured withglucose as the substrate in the presence of 4 m M CoCl2. The apoenzyme ... metal-containingenzymes, the apoenzyme was incubated for 2 h at roomtemperature with 5 m M metal chloride. The enzyme solutionwas then dialyzed once against 1 L 50 m M Mops (pH 7.0)to remove the metal ... and the growth temperature of the source organismwas observed: XIs from the most thermophilic sourcestypically had lower N þ Q content [23]. Of the XIsexamined, only the enzyme from the mesophilic...
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Báo cáo khoa học: A new rice zinc-finger protein binds to the O2S box of the a-amylase gene promoter pptx
... data indicate thatRAMY protein binds specifically to the O2S elemen t. Wehave also determined the importance of the amino acidswithin the binding domain of RAMY protein and analyzed the time ... GST-mutant RAMY fusionprotein (lanes 3–6), showing RAMY binding to DNA a nd the effects of mutations within the RAMY DNA-binding domain on the ability of the purified fusion protein G ST–RAMY to ... hybridization experiment suggests asingle copy of the RAMY gene is present in the ricegenome.RAMY contains a novel zinc fingerUsing the yeast one-hybrid system, the GAL4–RAMYfusion protein...
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Báo cáo Y học: Cellular stresses profoundly inhibit protein synthesis and modulate the states of phosphorylation of multiple translation factors potx
... translation in mammalian cellsinvolves the regulation of a range of components of the translational machinery, principally by changes in theirphosphorylation, leading to modulation of their activities ... SDS/polyacrylamide gels containing 15% acryla-mide/0.4% methylene bis-acrylamide. For analysis of eEF2,gels contained 10% (w/v) acrylamide plus 0.1% methylenebis-acrylamide. For analysis of the ... responds in the same way to all the stresses used,it seems likely that inhibition of eIF4E by increased bindingto 4E-BP1 represents a major mechanism, possibly the primary mechanism, by which these...
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Báo cáo Y học: Extra terminal residues have a profound effect on the folding and solubility of a Plasmodium falciparum sexual stage-specific protein over-expressed in Escherichia coli pptx
... properfolding or of increased solubility of the protein of interest.We propose that the success of fusion protein systemsshould be ascertained only once the protein of interest hasbeen cleaved off ... that the fusion proteins werelabile and only partially soluble in five of the constructsresulting in very poor yields. Intriguingly, in the sixth con-struct, the yield of soluble fusion protein ... were interested in producing large amounts of a 27Kcytoplasmic protein (Pfg27) from Plasmodium falciparumfor biochemical and biophysical studies. This protein plays acrucial role in the sexual...
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Báo cáo Y học: Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides pot
... a2b2c2composition. The K m for the threemain substrates were 1.6 m Mfor 1,2-propanediol, 5.5 m Mfor 1,2-ethanediol and 8.3 m Mfor glycerol. The enzymerequired the adenosylcobalamin coenzyme for catalyticactivity ... 5735metabolism is catalysed by a dehydratase that convertsglycerol to 3-hydroxypropionaldehyde, a precursor of acrolein [12]. The dehydratase enzyme plays therefore akey role in the development ... lachrymatorychemical generating a peppery flavour [15]. Acrolein is notissued from the bacterial metabolism but rather is in chemical equilibrium with 3-hydroxypropionaldehydeformed by the dehydratase...
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Báo cáo Y học: Amphibian peptides that inhibit neuronal nitric oxide synthase The isolation of lesueurin from the skin secretion of the Australian Stony Creek Frog Litoria lesueuri docx
... tructure of the calmodulin binding sites of any isoform beingreported. Thus, we cannot make meaningful comparisons of common structural motifs that might underlie calmodulinbinding to the e nzymes.Most ... further examine t he mode of action bywhich they were inhibiting nNOS. Lesueurin and citro-pin 1.1 (both from inhibitor group 1), f renatin 3 (inhibitor group 2) and c aerin 1 .9 (inhibitor group ... from changes in enzyme velocities in the presence of increasing amounts of the peptides citropin 1.1(A), lesueurin (B), caerin 1.9 (C)andfrenatin3(D). Enzyme k inetics data o btained in the absence of...
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Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
... [49,50].Interaction of the YopD peptide with LcrH In the yeast two hybrid assay, the C-terminal amphi-pathic domain of YopD was required for LcrH binding[13]. Because the YopD–LcrH complex is important ... domain of YopD. The peptidestructures were examined using CD spectroscopy and 2D homonuclear/heteronuclear NMR spectroscopy. Usingthese peptides, the interaction between the amphipathicdomain of ... amphipathic domain of YopD from YersiniaTobias Tengel1, Ingmar Sethson1and Matthew S. Francis21Departments of Organic Chemistry and2Molecular Biology, Umea˚University, Umea˚, SwedenTo...
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