... Proceedings of EACL '99
The GENIA project: corpus-based knowledge acquisition and
information extraction from genome research papers
Nigel Collier, Hyun Seok Park, Norihiro ... biochemical
information from journal papers and ab-
stracts. GENIA will be available over
the Internet and is designed to aid in
information extraction, retrieval and vi-...
...
tween
bandwidth
and
largeur de bande
can
be obtained through the edge linking these
two units (type 1), or through two edges,
one from
bandwidth
to
largeur de bande.,
and one from
bandwidth ...
edges from
bandwidth
to
largeur
and
bande
(type 3),
2. secondly, there may be conflicts between
connections: in figure 1 both
largeur de
bande
and
tdldcommunications...
... lCiÆmL
)1
,160mCiÆmmol
)1
)wasfromPerkinElmer
Life Sciences Inc. Restriction enzymes were from New
England Biolabs. Expand High Fidelity polymerase and
inorganic pyrophosphatase were from Roche. Nucleotri-
phosphates ... SerRS forms complexes with homologous and
heterologous archaeal tRNAs
Ser
and tRNAs
Sec
(lanes 3
and 4, 5 and 6, respectively) as well as with tRNA
Ser
substrat...
... Primers 1 and 3, primers 2 and 3, and primers 1
and 4 were used to amplify the genes encoding Tma-RNase
HI, Tma-CD and Tma-ND, respectively. The resultant
DNA fragments were digested with NdeI and ... analysis.
Acknowledgements
This work was supported in part by a Grant
(21380065) from the Ministry of Education, Culture,
Sports, Science, and Technology of Japan, and by an
In...
... distribution of the
enzyme and substrates.
Deduced from the crystal structure of pyridoxal kin-
ase from sheep [17], the hydrophobic and hydrophilic
regions of the substrate and cofactor binding domain
of ... solution and a 0.1 m ATP stock solu-
tion. The general incubation temperature was 37 °C. To
determine the temperature and pH optima, reactions were
performed in a range fr...
... related to
PAL2, and PAL3 always clusters together with PAL4
[61]. In A. thaliana, from the molecular phenotype,
common and specific functions of PAL1 and PAL2
were delineated, and PAL1 was qualified ... 266–295 (chain B) and 385–399 (chain C) in
SmPAL
out ⁄ in
and 33–128 and 333–351 (chain A), 272–303
(chain B) and 293–306 (chain C) in PlPAL
out ⁄ in
. The preop-
timizations...
... to
MBP were from New England Biolabs. Polyclonal anti-
bodies to the N-terminal region of RasGRF1 have been
previously described [28]. Chemicals were from Sigma
unless otherwise indicated.
Acknowledgements
We ... recep-
tors [20,21] and requires both calcium and calmodulin
to exert its activity on Ras [18,21]. The protein is phos-
phorylated on serine, threonine and tyrosine residue...
... RNase H activity are ubiquitous and have
been isolated from a variety of organisms, ranging from
viruses to prokaryotes and eukaryotes [3]. The best char-
acterized and f unctionally understood ... lLRNasin(20UálL
)1
;
Promega) and 5 lL cell extract, and incubated at 37 °Cfor
5 m in. RNA was subsequently precipitated in the presence
of glycogen, after removal of proteins by phen...
... ranging from 2.0 to 3.0 and from
1.4 to 1.8 with NADH and succinate, respectively,
used as substrates.
In a set of experiments, Rf (0.2–30 lm) was added
to purified intact TBY-2 mitochondria, and ... mitochondria from mammals and
yeasts [29–32]. Moreover, the data here reported imply
the existence of intramitochondrial enzymes that allow
for FMN and FAD synthesis starting from...
... subcloned from pSG5-T-H42Q (a gener-
ous gift from J. A. DeCaprio) to pGEM and subsequently
inserted to the EcoR1 and Sal1 sites of pGEX-4T-1. The
resulting plasmid was digested with Not1 and HindIII,
blunt ... DnaJs lack the cysteine
repeats and type III DnaJs lack both the G ⁄ F region
and the cysteine repeats. Type I and Type II proteins
seem to have similar functions and...