... cysteine proteasespapain, cathepsin L and cathepsin V using benzyloxycar-bonyl (Z)-Phe-Arg-7-amido-4-methylcoumarin (AMC) assubstrate, and for legumain with Z-Ala-Ala-Asn-AMC asthe substrate, while ... pooled,concentrated by ultrafiltration (Amicon UM-10; Millipore,Vienna, Austria) and dialyzed against 0.02 m Tris–HCl, pH7.5. The sample was then applied to a column of DEAE–Sephacel (Pharmacia-LKB, Uppsala, ... Nucleic Acids Res 36, D190–D195.13 Altschul SF, Madden TL, Schaffer AA, Zhang J, ZhangZ, Miller W & Lipman DJ (1997) Gapped BLAST andPSI-BLAST: a new generation of protein databasesearch...