... 5¢-TTCTTAACCATGGGCTTCAAAAACCTGAT-CTGG-3¢;BPL-rev,5¢-TTAAGGATCCTAAGAACGAGACAGGCTGAACTCTCC-3¢;BCCPD67, 5¢-GTAACCATGGGTGAACAGGAAGA A- 3¢;BCCP-rev,5¢-GGATCCTTAAACGTTTGTGTCTATAAG-3¢; BCCP K117L, 5¢-GAAGCTCTACTGGTTATGAAC-3¢.DNA was isolated from agarose using a ... details are as follows (restriction sitesare indicated by underlining and mutagenic changes areshown in bold). BPL-for, 5¢-TTCTTAACCATGGGCTTCAAAAACCTGAT-CTGG-3¢;BPL-rev,5¢-TTAAGGATCCTAAGAACGAGACAGGCTGAACTCTCC-3¢;BCCPD67, ... for DNAbinding; a central catalytic domain, which contains a highlyconserved GRGRRG motif shown to be involved in biotinbinding [11]; and a small C-terminal domain which has beenpostulated...