... localizedfashion as revealed by mutational and X-raycrystallographic analysesMuhammad S. Rohman1, Takashi Tadokoro1, Clement Angkawidjaja1, Yumi Abe1,Hiroyoshi Matsumura2,3, Yuichi Koga1, ... extension: tailor-made genes using thepolymerase chain reaction. Biotechniques 8, 528–535.34 Kanaya S, Katsuda C, Kimura S, Nakai T, Kitakuni E,Nakamura H, Katayanagi K, Morikawa K & IkeharaM ... Val and Lys90 fi Asn mutations; 5·-RNase HI, 4·-RNaseHI derivative with additional Arg97 fi Gly mutation; 6·-RNase HI, 5·-RNase HI derivative with additional Asp136 fi His mutation; D136H-RNase...