Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf
Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf
... likelycandidate for the catalytic glutamate of propionate CoA-transferase based on these data was glutamate 324 (Fig. 3 ).Detection of glutamate 324 as the catalyticcarboxylate of propionate CoA-transferase As ... Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site Thorsten Selmer, Angela Willanzheimer and Marc HetzelFB ... detected by the formation of stablederivatives of the catalytic glutamate and hydroxamicacids [15] or indirectly by oxygen exchange experiments[14,16].To date, only the crystal structure of glutaconate...
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Báo cáo Y học: Matrilysin (matrix metalloprotease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces docx
... processed by matrilysin. Theseactions of matrilysin may also contribute to the malig-nant growth of cancer cells. Understanding the patho-logical significance of the cleavage of annexin II and other ... represents the mean ± SD of triplicate assays. (C) Enzymatic activity of tPA bound to the cell surface. CaR-1 cells were treated with the indicated concentrations of matrilysin and then with tPA ... from iGENE (Tsukuba, Japan). Cells were inoculated on the daybefore transfection at a cell density of approximately 30%saturation in 24-well culture plates and treated with the siRNA or the control...
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Báo cáo khoa học: Structural diversity in lipopolysaccharide expression in nontypeable Haemophilus influenzae Identification of L-glycero -D-manno-heptose in the outer-core region in three clinical isolates potx
... structurally identical to the LPS of the clinical isolate, thereby facilitating analysis of the moreelongated Hep4-glycoforms.Methylation analysis of a dephosphorylated portion of the major ... todecrease the OS heterogeneity and thereby simplify the elucidation by NMR, OS-1¢ was repeatedly chromato-graphed on a P-4 column with intermediate selection for the Hep3- and Hep4-glycoforms (by ESI-MS). ... acetylation at O-4 of GlcI and at O-3 of HepIII, as observed for the Hep3-glycoforms (see above). From the combined data, the structure in Scheme 4 is proposed for the Hex5 glycoform in the Hep4-glycoform...
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Báo cáo y học: "Does switching from oral extended-release methylphenidate to the methylphenidate transdermal system affect health-related quality-of-life and medication satisfaction for children with attention-deficit/hyperactivity disorder&
... wear times of 4 and 6 hours, instead of the 9-hourwear time used in this study, suggests that some late dayside effects may be attenuated by early removal of the patch [25].Limitations The current ... tolerability of MTS, at study endpoint the majority of caregivers (59.6%)agreed that the study medication decreased their child'sappetite. The majority of caregivers (88.1%) disagreedthat the ... their child's socialinteractions since beginning the study medication, and 91.9% were satisfied with the efficacy of the study medi-cation on their child's attention. The majority...
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Báo cáo y học: "Pre-hospital intubation by anaesthesiologists in patients with severe trauma: an audit of a Norwegian helicopter emergency medical service"
... pre-hospital patient charts,as well as in-hospital data from the patient records. The data included the type of airway device and drugs used tofacilitate ETI, complications and the use of monitoring,including ... Norway, and its primary areas of operation are the mixed urban and rural districts of Rogaland County,which consist of just over 400,000 inhabitants. The medi-cal conditions treated by the HEMS ... man-agement) and whether ETI attempts were successful and without major complications (patient safety).Materials and methodsStavanger HEMS The Stavanger HEMS is part of the national HEMS system of Norway,...
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Báo cáo y học: "1, 25-dihydroxyvitamin D3 decreases adriamycin-induced podocyte apoptosis and loss"
... beneficial effects of 1,25(OH)2D3 on podocytes may be attributable to inhibit podocyte apoptosis and the amelioration of podocytopenia. Key words: 1, 25 -dihydroxyvitamin D3, podoc yte, proteinuria ... indicate that 1,25(OH)2D3 can decrease po-docyte loss and inhibit podocyte hypertrophy in subtotally nephrectomized rats (8). In puromycin aminonucleoside nephropathy rats, p o d o c y t ... podocyte apoptosis and podocyto-penia. The present study aimed to investigate the ef-fects of 1,25-(OH)2D3 on podocyte apoptosis and po-docytopenia induced by adriamycin (ADR) and the potential...
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Báo cáo y học: "Line bisection performance in patients with generalized anxiety disorder and treatment-resistant depressionLine bisection performance in patients with generalized anxiety disorder and treatment-resistant depression"
... functions that contribute to these pathologies on the one hand, and probably help to further address the overlaps between anxiety and depression on the other. Bearing that GAD and TRD patients often ... of another scale of dissi-mulation (infrequency or lie) were randomly inserted into the test body. Any score above 3 on the infre-quency scale suggests either inattention to the content of ... Department of Clinical Psychology and Psychiatry, Zhejiang University School of Medicine, Hangzhou, China; 3. Department of Psychiatry, Second Hospital, Zhejiang University School of Medicine,...
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Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
... of this enzyme apart from modulation of GS by NO. Activity of the GS could be involved in the regulation of concentration of glutamate in the ex-tra-cellular space of neurons with other systems, ... this study clearly demonstrated the increased formation of NO and supports the in-volvement of NO in the pathophysiology of anoxia (hypobaric hypoxia) and reperfusion damage in brain. The increased ... as-troglial glutamate transporter-I (GLT-1), and also mi-croglial antiporter for cystine and glutamate which may release glutamate when the demand of gluta-thione synthesis was increased by oxidative...
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Báo cáo y học: "Association between regulated upon activation, normal T cells expressed and secreted (RANTES) -28C/G polymorphism and asthma risk – A Meta-Analysis"
... indicated that genetic factors may play an important role in the pathogenesis of asthma. Regulated upon activation, normal T-cell ex-pressed and secreted (RANTES), which generated by T lymphocyte, ... name, year of publica-tion, country of origin, ethnicity, type of asthma, number of cases and controls, and the minor allele fre-quency in controls. Meta-Analysis. The risk of asthma associated ... significantly increased risk of asthma (GG+CG vs CC: OR=1.24, 95%CI=1.08-1.41) without any be-tween-study heterogeneity. In the stratified analysis by asthma type, age and ethnicity, we found that the...
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Báo cáo y học: "TPO, but not soluble-IL-6 receptor, levels increase after anagrelide treatment of thrombocythemia in chronic myeloproliferative disorders"
... that serum levels of TPO, the major cytokine involved in the regulation of mega-karyocyte and platelet growth and release, was raised at six months of anagrelide treatment; concomitantly there ... blood platelet counts for the 45 patients of the ET and the PV groups are given in Table 1, together with the platelet counts at 6 months. All 45 patients displayed a similar drop of the platelet ... chronic mye-loproliferative disorders (MPD) is thrombocythemia, always present in essential thrombocythemia (ET) and often in polycythemia vera (PV), myelofibrosis (MF) and chronic myelogenous...
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