Báo cáo khoa học: The three typical aspartic proteinase genes of Arabidopsis thaliana are differentially expressed docx

... whether the aspartic proteinase genes are also expressed in the guardcells of leaves; if so, this could explain the greater expression of the AtPaspA1 gene observed in the light. The aspartic proteinases ... glycosylation; proteinase. We have been studying the aspartic proteinase genes in Arabidopsis with the goal of understanding their function in the whole plant. Aspartic proteinases (EC 3.4.23) are one of the ... cathepsin D gene. The top line of the ﬁgure shows the arrangement of the functional regions of the plant aspartic proteinase genes like in Fig. 1. Below that are the representative sequences of...

Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx

... considered to display differences in the fold of the main chains. None of these includes any of the binding sites, but some are located in the vicinity. Three of the regions with different conformationinvolve ... similararrangement, and the side chain of Ser97 in VPRK istoo short. One side of the loop is involved in the bind-ing of the P2–P4 residues of a substrate. The other side of the loop is close to Arg94 ... different fromboth of the other two enzymes is after residue 213,where two residues are inserted. The length of the loopcomprising residues 16–29 is identical in the three enzymes, but the conformation...

Tài liệu Báo cáo khóa học: The PAS fold A redeﬁnition of the PAS domain based upon structural prediction ppt

... Toovercome the fact that the prosa z-score is dependant of the length of the amino acid sequence, the z-score wasnormalized using the natural logarithm of the sequencelength [39]. The resulting ... helix and the FG loop correspond to that region of the PAS fold that is part of the region which tethers the PAS Table 4. Backbone root mean square deviation values (in A˚ngstrom) of the structural ... all PAC-annotated A. thaliana proteins contain a PAS domain.Therefore, in the case of A. thaliana, thePASandPACmotifs are inseparable, indicating that the annotation of these proteins as containing...

Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot

... changed either in the presence orabsence of the metal cofactor. By contrast, the sub-strate binding afﬁnity of the RNase H domain proba-bly increases in the presence of the metal cofactorcompared ... alter the interaction between the sub-strate and metal ion, such that the scissile phosphategroup of the substrate and the Mg2+ion are arranged ideally. The effect of this alteration on the coordination ... concentrations of the pro-teins, the KDvalue was determined using the steady-stateafﬁnity software available in biaevaluation (Biacore).Thermal denaturationThermal denaturation curves of the proteins...

Báo cáo khoa học: The product chain length determination mechanism of type II geranylgeranyl diphosphate synthase requires subunit interaction pptx

... phenylalanine 108 suppliedfrom the other subunit exists in the proximity of the x-end [24].These results led to the hypothesis that the prenyl-chain of the product elongates in the space enclosed bya-helices ... in the center of the cavity and likely to act as the bottom of it in thesestructures; however, the structural information is inde-cisive with respect to the role of the residue because the ... Closeview of the substrate pocket of S. alba GGPS (left) and that of the modeled dimeric structure of P. ananatis GGPS (right). The model of P. ananatis GGPS was constructed based on the crystal...

Báo cáo khoa học: The human pyridoxal kinase, a plausible target for ginkgotoxin from Ginkgo biloba docx

... deter-mining the kinetic features of PKH. However, the datafor the catalytic efficiency of ginkgotoxin and DPN are of the same order of magnitude and reflect their antivita-min character, as they are ... the antivitamin character of both compoundsand explains a depletion of cofactor formation in the organism in the presence of the antivitamins. The kinetic data (Table 1) are a reflection of the structural ... reported [24]. The authors of these case studiesassume that the occurrence of the epileptic convulsionswas due to the ingestion of Ginkgo remedies. However,they neither specify the respective...

Báo cáo khoa học: The structural basis for catalytic function of GMD and RMD, two closely related enzymes from the GDP-D-rhamnose biosynthesis pathway pdf

... positions of the corresponding side chain, Arg220, the NADPH cofactor and the substrate analog hexosein the MUR1 structure (Fig. 11). Some of the distancesbetween these groups are rather long ... Stereoview of the RMD monomer. The cofactor-binding domain and the substrate-binding domain are shown in aqua and light sand, respectively; the APPR portion of the cofactor (dark gray) and the ligand ... search (using the blastp algorithm[37]) of the P. aeruginosa RMD sequence, however, 89 of the top 100 hits had glutamine in this position; 10 of the others had arginine in its place, and the ﬁnalvariant...

Báo cáo khoa học: The consensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system ppt

... the relationship between the efﬁciency of protein N-myristoylation and the identity of the amino acids at positions 3, 6 and 7 of the con-sensus motif in plant target proteins with the use of ... proteins of the expected size. The positions of pro-tein size standards are shown on the left of the gel.Table 1. Observed and calculated mass values for the trypticN-terminal peptides of ARF1A1c ... 3, the prediction program gave low scores for the possibility of N-myristoylation of these constructs(Table S1). We further selected eight A. thaliana pro-teins on the basis of combinations of...

Báo cáo khoa học: The cellular oxygen tension regulates expression of the endoplasmic oxidoreductase ERO1-La ppt

... the expression of the rat,mouse and human homologues of ERO1-Like protein a butnot of the isoform ERO1-Lb are stimulated by hypoxia inrats vivo and in rat, mouse and human cell cultures. The temporal ... speculate from these ﬁndings that the expression of ERO1-La is probably regulated via the HIF-pathway and thus belongs to the family of classicoxygen regulated genes. Activation of the unfolded ... : 1000) of a cDNAgenerated from the pooled RNA of the normoxic (control)cultures (time 0) of the respective experimental series(standard cDNA). Analysis of the individual unknownstherefore...

Tài liệu Báo cáo khóa học: The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life pptx

... todetermine to what extent they follow the rules accountingforidentitiesinprokaryotesandeukaryotes[1,4,5].Inspite of the universality of the genetic code, there are oftenbarriers to aminoacylation ... accepted 1.1 nmol of serine per A260unit of tRNA. The following archaeal tRNAs were prepared byin vitro transcription [20] of their synthetic genes, constructedaccording to the published sequences ... number of unpaired nucleotides at the base of variable arm reﬂects the similarity to eukaryotictRNAs, due to the presence of at least one unpairednucleotide between the possible stem of the variable...

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