Báo cáo khoa học: Engineering of a monomeric and low-glycosylated form of human butyrylcholinesterase pdf
Tài liệu Báo cáo khoa học: Stefin A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft doc
... loop residues adaptsto each binding ligand in its own way and swings outonly as much as is mandatory.Results and DiscussionCrystals of the complex of stefin A and cathepsin Bcontain complete ... red).Table 1. Average distances between CA atoms of the stefins and catalytic residues of cysteine proteases.Distance calculated d (A ˚)Papain–stefin B 23.93Cathepsin H–stefin A 23.36 ± 0.23Cathepsin ... this, we calculated the aver-age distances between CA atoms of the active site cys-teine and histidine residues in cathepsins B and H and the center of CA atoms of stefins in the structures of both...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan, ROC2 Institute of Physics, Academia Sinica, Taipei, Taiwan, ROC3 Department of Biochemistry, University of Minnesota College of ... 7.0) at a concentration of 0.5 mgÆmL)1. Spec-tra were obtained as the average of five successivescans with a bandwidth of 1.0 nm and a scan speed of 20 nmÆmin)1.Steady-state tryptophan fluorescencemeasurementsMeasurements ... Salmon testesDNA and some analytical grade chemicals such as EDTA,Tris ⁄ HCl, CaCl2, NaCl and mineral oil were obtained fromSigma (St Louis, MO, USA). Salmon testes DNA for theenzyme activity...
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Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot
... CAF-1 – a molecular link betweenrecombination and chromatin assembly during meiosisSatomi Ishii*,†, Akiyo Koshiyama*, Fumika N. Hamada, Takayuki Y. Nara, Kazuki Iwabata,Kengo Sakaguchi and ... 5¢-CGGGATCCATGTCGGGAGCAGATTCA; 381R, 5¢-TGCTACTTCTCTCAGCGGCCGCATTCTTAT. CcCac1L-C: 382F, 5¢-CATGCCATGGTGTCAGGGGATGTAGAAATG; 812R,5¢-GAGATTTCAGTTTCGTCACTCGAGCGG. To over-express N-terminal hexahistidine-tagged ... acids) and ED (glutamate/aspartate-rich; 522–578 amino acids) domains in CcCac1L areconserved amongst human and S. cerevisiae homo-logues (Fig. 1A) . The KER and ED domains areknown to interact...
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Tài liệu Báo cáo khoa học: "Word representations: A simple and general method for semi-supervised learning" doc
... the final NER F1 results. We compareto the state -of- the-art methods of Ando and Zhang(2005), Suzuki and Isozaki (2008), and forNER—Lin and Wu (2009). Tables 2 and 3 showthat accuracy can be ... reduce data sparsityin the labeled training data, semi-supervisedapproaches improve generalization accuracy.Semi-supervised models such as Ando and Zhang(2005), Suzuki and Isozaki (2008), and ... Suzukiet al. (2009) achieve state -of- the-art accuracy.However, these approaches dictate a particularchoice of model and training regime. It can betricky and time-consuming to adapt an existing...
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Tài liệu Báo cáo khoa học: "What lies beneath: Semantic and syntactic analysis of manually reconstructed spontaneous speech" pdf
... utter-ances (about 72% of the annotated SSR data) canbe given a semantic analysis in the following sec-tions. For each well-formed and grammatical sen-tence, all (non-auxiliary and non-modal) ... Proceedings of the 47th Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 746–754,Suntec, Singapore, 2-7 August 2009.c2009 ACL and AFNLPWhat lies beneath: Semantic and syntactic analysis of ... of 54 neutral argument nodes directly as-signed as semantic roles were ARG1, and another33.3% were ARG0. Nearly a quarter of insertedarguments became part of a larger phrase serv-ing as a...
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Tài liệu Báo cáo khoa học: "Discourse Relations: A Structural and Presuppositional Account Using Lexicalised TAG*" docx
... Thus, associated with the derivation of (2c) are the assertions that the situation associated with B is a cause for that associated with A and that the situ- ation associated with B is one of a ... work to date surely shows the benefit of an approach that narrows the gap between dis- course syntax and semantics and that of the clause. References Nicholas Asher and Alex Lascarides. 1999. ... the work of Grote (1998), Grote et al. (1997), Jayez and Rossari (1998) and Lagerwerf (1998). Secondly, the approach demands an understand- ing of the attentional characteristics of presupposi-...
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Tài liệu Báo cáo khoa học: "Prosodic Aids to Syntactic and Semantic Analysis of Spoken English" ppt
... the range of syntactic pos- sibilities and the parser will align tone group and move syntactic boundaries at a later stage. By integrating syntax and semantics, the Parser is capable of resolving ... terms of available system functions. A dia- logue manager manages interaction with the speaker and retrieves database information, 3. PROSODY EXTRACTION As the input to the parser is spoken language, ... Prosodic Aids to Syntactic and Semantic Analysis of Spoken English Chris Rowles and Xiuming Huang AI Systems Section Australia and Overseas Telecommunications Corporation Telecommunications Research...
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Báo cáo khoa học: Regulated expression by PPARa and unique localization of 17b-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver pdf
... Onoduka J, Homma K, Yamaguchi S,Mori M, Higashi Y, Makita M, Kinoshita T, Noda J,Itabe H et al. (2006) Long-chain fatty acids induce lipiddroplet formation in a cultured human hepatocyte in a manner ... 3,17-androstanediol and abundant expression insteroidogenic tissues such as placenta and gonads havebeen described [7]. 17b-HSD11 also has a proteindomain of glucose ⁄ ribitol dehydrogenase, and ... one containing a PPARa ligand, Wy-14 643(–), were separated by SDS ⁄ PAGE and analyzed by western blot-ting. After analysis, the transferred membrane was stained withCoomassie Brilliant Blue...
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Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt
... membrane and periplasmic space, whereas PilM, PilN and PilO are inner membrane proteins that probably form part of the assembly platform and are involved in the assembly of the DNA translocatorcomplex ... membrane, whereas PilW, PilQ and PilA4 are located in the inner and outer membranes. These data show thatPilMNOWQ and PilA4 are components of a DNA translocator structurethat spans the inner and outer ... proteinsPilMNOWQ and PilA4, and demonstrate that the pilMNOWQ genes areeach essential for natural transformation. We identified three differentforms of PilA4, one with an apparent molecular mass of 14 kDa,...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
... Huey-Jen Fang1 and Tian-Yow Tsong2,31 Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan2 Institute of Physics, Academia Sinica, Taipei, Taiwan3 Department of Biochemistry, ... K110 and K133. Other chargedamino acids such as D73, D83 and E101 reinforce the interactions of E75 and E129.H M. Chen et al. Local stability and key acidic amino acids in staphylococcal nucleaseFEBS ... USB (Cleveland, OH). Salmon testes DNA and some analytical grade chemicals such as EDTA, Tris ⁄ HCl,CaCl2, NaCl and mineral oil were from Sigma (St Louis,MO, USA). Salmon testes DNA applied...
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