Báo cáo Y học: The membrane-bound [NiFe]-hydrogenase (Ech) f

Báo cáo Y học: The membrane-bound [NiFe]-hydrogenase (Ech) from Methanosarcina barkeri : unusual properties of the iron-sulphur clusters docx

... redox properties of [NiFe]-hydrogenases [27,41]. The interaction of H 2 with hydrogenases offers the possibility to study redox changes in enzyme in the absence of redox mediators simply by varying ... for the catalytic action of [NiFe]-hydrogenases [7]. The study of hydrogenases in methanogens led to the discovery of a third class of hydrogenases, not containing a...

Ngày tải lên : 17/03/2014, 10:20

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Báo cáo Y học: Brassica napus soluble epoxide hydrolase (BNSEH1) Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris docx

... recovered from the afﬁnity chromatography displayed activity and a symmetrical active peak was obtained upon gel ﬁltration analysis of the native enzyme. Of the plant sEH sequences available in the ... inhibitory effects on the hydrolysis of TSO by the recombinant enzyme (Fig. 5). N,N¢-dicyclohexylcarbodiimide was found to be a potent inhibitor and 10 l M of this compoun...

Ngày tải lên : 31/03/2014, 08:20

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Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx

... in exploring the regulatory behavior of this enzyme. To avoid misinterpretation of the results, the composition and stoichiometry of the bound cofactor(s) and the stability of the dimeric structure of ... composition of cofactor, the catalytic sites of epimerase remain indistinguish- able. Stability of subunits According to Scheme 1, the subunits of epimerase...

Ngày tải lên : 16/03/2014, 00:20

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Báo cáo y học: "Parvovirus B19 Genotype Specific Amino Acid Substitution in NS1 Reduces the Protein’s Cytotoxicity in Culture"

... results support the hypothesis that in the setting of persistent B19 infection, direct B19 NS1-induced cytotoxicity may play a role in idiopathic fulminant liver failure. Key words: Parvovirus, ... However, even though the primary structure is not telling, the contribution of this residue to the overall structure of the enzymatic folds can be pro- posed. The change f...

Ngày tải lên : 26/10/2012, 09:32

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Báo cáo y học: "Elevated plasma homocysteine is positively associated with age independent of C677T mutation of the methylenetetrahydrofolate reductase gene in selected Egyptian subjects"

... genotypes and the result of the Chi square testing in the different studied groups. Showing the frequency and the results of the cross tabulation of the T allele and the different genotypes in the ... of cytosine (C) by thymine (T) at nucleotide 677 of the MTHFR gene that converts an alanine to a valine residue was responsible for the thermolability of MTH...

Ngày tải lên : 03/11/2012, 09:49

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Tài liệu Báo cáo Y học: Trehalose-based oligosaccharides isolated from the cytoplasm of Mycobacterium smegmatis Relation to trehalose-based oligosaccharides attached to lipid docx

... chromatography in the following solvent sys- tems: butan-1-ol/pyridine/0.1 M HCl ( 5:3 :2 , by vol.); propan-2-ol/butan-1-ol/water (140 : 20 : 40, by vol.); pro- panol/ethyl acetate/water (140 : 20 : 40, by ... allow chemical characterization of each. CO3-1 was identiﬁed as trehalose by TLC as well as by MALDI-TOF MS analysis of the products obtained by permethylati...

Ngày tải lên : 22/02/2014, 07:20

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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt

... followed the kinetics of amylose synthesis over a 5-day period of nitrogen starvation and measured the amounts of starch, amylose, the k max of the starch fractions, the degree of crystallinity and the ... course of storage starch synthesis show that amylopectin and amylose synthesis are partly disconnected and that amylose synthesis persists when the rate of polys...

Ngày tải lên : 22/02/2014, 07:20

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Báo cáo Y học: Amino acids 3–13 and amino acids in and ﬂanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf

... AR 3)13 deletion, whereas in the mammalian assay almost all reporter gene activity was lost. The most obvious difference between both assays is the coupling of AR.NTD to GalAD in the yeast assay, and the absence of a ... regulation of AR function. Detailed knowledge on the role of the AR N/C interaction would require the elucidation of its function under more physio-...

Ngày tải lên : 17/03/2014, 10:20

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Báo cáo Y học: Guanosine diphosphate-4-keto-6-deoxy-D-mannose reductase in the pathway for the synthesis of GDP-6-deoxy-D-talose in Actinobacillus actinomycetemcomitans potx

... be synthesized by another GDP-4-keto-6-deoxy- D -mannose reductase and the stereoselectivity of the reduction deter- mines the direction of synthesis of these two 6-deoxyhex- oses. However, neither ... respectively) to the proteins enco- ded by ORFs in the clusters responsible for synthesizing the SPAs in other serotypes of A. actinomycetemcomitans. The biosynthetic pa...

Ngày tải lên : 17/03/2014, 10:20

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Báo cáo Y học: A new high afﬁnity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor potx

... 0.12 pY429pY431 1.1 ± 0.03 F429pY431 4.6 ± 0.02 pY429F431 4.9 ± 0.02 Y4 2 9Y4 31 ND pY443 ND pY461pY464 ND Y4 61pY464 ND pY46 1Y4 64 ND pY479 ND Ó FEBS 2002 SOCS-3 binds to the pY429pY431 motif of Epo-R (Eur. J. ... specifically interact with the tyrosine motifs pY401 and pY429pY431. pY429pY431 was more potently recruiting SOCS-3 than pY401, reflecting the high affinity binding determi...

Ngày tải lên : 24/03/2014, 00:21

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