Báo cáo Y học: Domain V of m-calpain shows the potential to form an oblique-orientated a-helix, which may modulate the enzyme’s activity via interactions with anionic lipid potx
Báo cáo Y học: Domain V of m-calpain shows the potential to form an oblique-orientated a-helix, which may modulate the enzyme’s activity via interactions with anionic lipid potx
... Domain V of m-calpain shows the potential to form an oblique-orientated a-helix, which may modulate the enzyme’s activity via interactions with anionic lipid Klaus Brandenburg1, ... respectively [2,3]. Theseobservations suggested that other factors may be involved in the activation of m-calpain because, clearly, the millimolarlevels of Ca2+needed to activate the enzyme in vivo ... domainalorganization, with the larger subunit divided into domains I to IV and the smaller subunit divided into domains V andVI. Domain II possesses the active site and is a papain-likecysteine protease domain, ...
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Báo cáo Y học: Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y pot
... as to why C34 1V exhibits an increasedDGàcompared with the wild-type enzyme. As the properties of Val, such as size and hydrophobicity, are similar to those of Cys and the affinity of C34 1V for ... Single bands onSDS/PAGE analysis verified the purity of all mutants. The protein yields of C341G, C341S and C341D were similar to that of the wild-type, whereas the yields of C34 1V, C341Fand C341H ... transformed by the method of Hanahan [16] using a standard transformation buffer.Purification of wild-type and mutant forms of carboxypeptidase Y Typical purifications were carried out as previously...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... together the substrate-binding and catalytic functions in proximity foreffective enzyme activity. Two LPL mutants lacking the lipase activity – one acatalytic site mutant (S132T) and the other ... cotransfection of S132T and WWW mutants. The lipase andesterase activities after transfection of vectorswere assayed (B). Gray bar indicates the recovery in LPL activity after cotransfection of ... structures of LPLin head -to- tail configuration after cotransfec-tion of inactive LPL mutants and recovery of catalytic activity (A). Lipase activity is notdetected in COS7 cells transfected with onlythe...
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Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx
... neurotoxic.Alternatively, APP can be hydrolyzed by the b secretase activity at the N-terminus of Ab, which is successivelyreleased by the c secretase after cleavage within the membrane [6] either ... that the mechanism of membraneinteraction and destabilization is different in the case of Ab,but it is fair to say that the similarity with the fusion domain of a virus is strongly suggestive of ... According to this view, which is supportedby the results of in vitro electrophysiologic measurements, the a-helical conformation of the peptide would induceformation of membrane channels, allowing the...
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Báo cáo Y học: Solution structure of the HIV gp120 C5 Domain pptx
... (1996) Anti-HIV activity of multibranched peptideconstructs derived either from the cleavage sequence or from the transmembrane domain (gp41) of the human immunodeficiencyvirus type 1 envelope. Virology ... Importantly, the C5 domain employed in the present study does not possess any tryptophan residues.Therefore, we performed fluorescence titrations of the HIVgp41 ectodomain in the presence of trifluoroethanol ... study(amino acids VKI) are observed in the gp120 X-raystructure; thus, the C5 domain can be overlaid with the Cterminus of the gp120 X-ray structure (the RMSD of the NMR VKI backbone to the...
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Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx
... from the human H407A mutant, where the basal activity increased dramatically, and the mouse Q404Hmutant, where the basal activity decreased by more than50% (Fig. 4). The basal activity of H407Q ... receptors we may anticipate amore detailed dynamic picture of the transition from a silent to an activated nuclear receptor and the role of heterodimerformation and coactivators in the relay of ... effect of PCN on Q404H should be viewed inperspective of the basal activity. The full activation of the mutant is similar in level to the wild-type in absolute terms,but as the basal activity is...
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Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf
... does have some interaction with the N-terminal domain, the mechanism of regulating the catalytic activity may be similar to that of the dimer. Inother word, the active site would be physically blocked ... Guo1, Yiwei Liu1, Yixin Sun1, Yu Gao1,2,Sheng Ye1, Guangtao Li2, Zihe Rao1, Boqin Qiang2and Jiangang Yuan21Laboratory of Structural Biology and the MOE Laboratory of Protein ... pairs of hydrogen bonds are formed in the active site of hTRXL-N (Fig. 4), accounting for the compactness and stability of the active site. The H-bondlength between the carbonyl oxygen of Cys34 and...
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Báo cáo Y học: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIa doc
... [44]. The presence of the charges in the C-terminal face of RIIaD/D may be essential to provide flexibility for the transition of the inactive holoenzyme (with bound catalytic subunit) to active form ... of the polar side chains of Gln23 and Gln24 in the top-bottom compared to the top-top structure. These observations, together with the exper-imental restraint violations, suggest that the top-bottomstructure ... Comparison of the ensembles of top-top and top-bottom struc-tures.6(A) Tube representation of the lowest energy structures of the top-top ensemble (red) and the top-bottom ensemble (blue) in the ordered...
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Báo cáo Y học: Transcriptional regulation of erythropoiesis Fine tuning of combinatorial multi-domain elements ppt
... Israel;2Department of Hematology, The Tel Aviv Sourasky Medical Center, Tel Aviv and Tel Aviv University, IsraelHaematopoiesis, the differentiation of haematopoietic stemcells and progenitors into various ... consequences, which together confirm the vital role played by specific domains in the corresponding transcription factors during in vivoerythroid and megakaryocyte development.Erythroid Kruppel-like factor ... erythroid progenitors. Polycytemia vera is a clonalmyeloproliferative disorder, leading to hyperproliferation of erythroid, myeloid and megakaryocytic cells. Sixty percent of polycytemia vera...
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Báo cáo Y học: Domain organization, folding and stability of bacteriophage T4 fibritin, a segmented coiled-coil protein docx
... the unit-volume heat capacity of water (calÆdeg)1ÆmL)1) (stored in Origin). The thermalcoefficient of cubic expansion of tantalum is 0.00002. The values of the van’t Hoff enthalpy of the process ... helicity at this temperature and changes in the magnitude of the accompanying enthalpy. The ratio o f the van’t Hoff enthalpy to calorimetric enthalpy of 0.39indicates that the nine putative segments ... [20].RESULTSEngineering and properties of fibritin deletion mutants To investigate the stability and thermodynamic properties of T4 fibritin, a set of recombinant truncated mutants wasdesigned and analysed. All these...
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