Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf

... 2003 RNA- binding of a PPR protein (Eur. J. Biochem. 270) 4081 RNA- binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA Takahiro Nakamura1, Karin ... bettercharacterize the RNA- binding properties and the possible protein protein interactions of HCF152, we produced the protein and several truncated versions in bacteria, andanalyzed its protein protein interactions ... contribution of the PPR motifs to the RNA- binding properties as the deletion of seven out of nine did not change the RNA- binding properties [41]. Deﬁning the exact structure of the HCF152 homodimer together...

Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt

... detailedcharacterization of amylose synthesis both in vivo and in vitro. We show that GBSSI is capable of synthesizing asignificant number of crystalline structures within starch.Quantifications of amount and type of ... rate of amylopectin synthesis. The increase in crystallinity wit-nessed during these first 12 h is in line with the high ratesobserved for amylopectin synthesis. However between 12and 58 h the ... fused into a network.This demonstrates that at least part of the synthesis canoccur at the surface of the granule. The apparent polarizedgrowth of the granules is consistent with either an aniso-tropic...

Báo cáo khoa học: hhLIM is a novel F-actin binding protein involved in actin cytoskeleton remodeling ppt

... includedSM22a, a known actin cross-linking protein, and BSA,which does not interact with or cross-link actin. In the absence of hhLIM, the majority of actin remained in the supernatant (S) and only a small ... dis-ruption of the second LIM domain of hhLIM abol-ished F-actin-binding activity, indicating the importantrole that LIM domain 2 plays in the control of assem-bly and organization of the actin cytoskeleton. In ... transport and cell growth, signaling, anddivision. Many of the actin-binding proteins affect the cytoskeletal structure and architecture by mediating the association of actin ﬁlaments into cables and...

Báo cáo khoa học: Relaxin-3⁄ insulin-like peptide 7, a neuropeptide involved in the stress response and food intake Masaki Tanaka pptx

... minireview, the expression of relaxin-3 in the brain, and particularly its functions, including the stress response and food intake, are described.Expression of relaxin-3 in the brainRelaxin-3 ... expressed in the brain and testis. In rodent brain, ana-tomical studies have revealed its predominant expression in neurons of the nucleus incertus of the dorsal pons, and a few other regions of the ... relaxin proteins involved in the growth and remodeling of reproductiveand other tissues during pregnancy [10]. In the mouse and rat brain, relaxin-3 expression wasreported to be localized to the...

Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt

... Ca2+.Removing the proposed Ca2+-coordinating residues in positions 1 and 3 of the EF-hand motif significantlycompromised the metal-binding capability of the engi-neered protein, indicating that the ... of investigating the Ca2+-binding sites in native Ca2+-binding proteins, weestablished a grafting approach to dissect their site-specific properties. This approach has been used in the investigation ... EF-hand protein domains. Our laboratory has developed a graftingapproach to probe the site-specific Ca2+-binding affini-ties and metal-binding properties of CaM [14] andother EF-hand proteins,...

Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform speciﬁcity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx

... coordination of the Zn(II)ion bound at the C-terminal moiety of CeMT1 is con-tributed to by histidines, and the number of these involved in metal binding is analyzed below.Thus, the overall ... at all on the bind-ing abilities of the Crs5 MT of S. cerevisiae [12], we decidedto continue our studies with the obtained clones.Recombinant synthesis and puriﬁcation of the C. elegans MT1 ... considered the study of the C. elegans MT system at the protein function levelto be of the highest interest, in order to shed light on the possible physiological functions of MTs in thisorganism and...

Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s ﬂuorescent probe for the determination of glutathione doc

... proceed for 10 min,and the ﬂuorescence was then measured at room tem-perature. In uence of interference The in uence of the main constituents of plasma on the determination of 2.00 · 10)6molÆL)1GSH ... solution and showed high bindingability because of the two adjacent bis(b-CD)s.Because of the conformation of the linker of 1, the nitrogen atoms and amido bond formed two chelaterings to coordinate ... reagentblanks, and S is the slope of the standard curve. The relative standard deviation was 2.5%, obtained from aseries of 11 standards each containing 2.00 lm GSH.When the concentration of GSH...

Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the speciﬁcity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf

... its S1¢ binding subsite. Substrate bindinginduces conformational changes involving the ArgA145-PheA146 fragment of the protein chain, acting as a ﬂap, ﬁrstopening and then closing the groove ... X-raycoordinates of amino acid residues in penicillinacylase [19] and AM1 docking calculations. The three N atoms of the side chain d-guanidinogroup of this residue are involved in H-bonding as ... with the O atom of the main chain CO group of the TrpB240;Ng1shares H bonding with O atom of the COgroup of LeuB387 together with O atom of c-OH of SerB386; Ng2is in H bonding withOd1of...

Báo cáo khoa học: Cofactor-independent oxygenation reactions catalyzed by soluble methane monooxygenase at the surface of a modiﬁed gold electrode pot

... H2O The regulatory protein (MMOB) plays several roles in the catalytic process including modulating the rate of electrontransfer between MMOR and MMOH [4,5], altering the redox potential of the ... to glutathione S-transferase and prepared the recombinant truncate by the same method used for the full-length protein. Like the recombinant intact MMOB[11], the recombinant MMOBtruhad two ... ventilated bymeans of a stream of air from an electric fan. The effect of the substrate methane on the electrochemical properties of the MMOH/MMOB/catalase system was investigated byenclosing the electrode...

Báo cáo khoa học: "PCFGs, Topic Models, Adaptor Grammars and Learning Topical Collocations and the Structure of Proper Names" ppt

... way of estimating LDA topic models.Current Bayesian PCFG inference algorithms re-quire time proportional to the cube of the length of the longest string in the training corpus, and sincethese ... generated by such aCFG. The relationship between the LDA modeland the PCFG can be understood by studying the trees generated by the CFG. In these trees the left-branching spine of nodes labelled ... Topici(one per word in the docu-ment) indicate the distribution of topics in the doc-ument. The corresponding Bayesian PCFG associatesprobabilities with each of the rules in the CFG. The probabilities...

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