... perwell) for expression of the GAL4 DNA-binding domain ( DNA-BD) f usio n p rotein ,together with the l uciferase repo rter plasmidpG5luc carrying five GAL4-binding sites up-stream of the TAT A ... mutant proteins (including VP16-fusedprotein) and luciferase protein, as a control product,were synthesized by using the TNT Quick CoupledTranscription/Translation S ystem (Promega). T he expres-sion ... K.,Yoshida,Y.,Sasabe,E.,Tsutsumi,S.,Hamada,T.,Honma,S.,Honma, K. & Kato, Y. (2004) Rhythmic expression of DEC1 and DEC2 in peripheral t issues: DEC2 is a potent suppressor forhepatic cytochrome P4 50s opposing DBP. Genes Cells 9, 317–329.14....