... each lane and analyzed on16% polyacrylamide gels using Coomassie Blue stain-ing. Lane S1, E. lagascae SCP2 Lys28Glu; lane S2,E. lagascae SCP2 Leu99Met; lane S3, E. lagascaeSCP2 Leu13Met ⁄ Leu14Met; ... Leu14Met; lane S4, A. thaliana SCP2Met100Leu; lane S5, A. thaliana SCP2 Met14Leu ⁄Met15Leu ⁄ His18Phe; lane S6, E. lagascae SCP2 wild-type; lane S7, A. thaliana SCP2 wild-type; lane M,PageRulerÔ ... compilation ª 2006 FEBSacid (DMPA). Ergosterol, sitosterol and cholesterolonly showed a marginal competing effect, and sterylglucoside, trimyristin, monogalactosyldiacylglycerol,galactosylceramide...