Báo cáo khoa học: Thermal behaviour of cubic phases rich in 1-monooleoyl-rac-glycerol in the ternary system docx
Báo cáo khoa học: Thermal behaviour of cubic phases rich in 1-monooleoyl-rac-glycerol in the ternary system docx
... Within a region of a cubic phase in the diagram an increase in the temperaturegenerally results in a decrease in the size of the unit cell.This is interpreted to be an effect of an increase in ... than obtained from the heating scan, againpointing to the difficulty of obtaining a true thermodynamicequilibrium for bicontinuous cubic phases. Fig. 4. Diffractogram of sample A showing (A) without ... report, the thermal behaviour of such cubic phases rich in MO has been investigated by X-ray diffrac-tion. The study comprises both one- and two-phase regions,including cubic phases in equilibrium...
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Tài liệu Báo cáo khoa học: Thermal unfolding of smooth muscle and nonmuscle tropomyosin a-homodimers with alternatively spliced exons docx
... studies of the thermal unfolding of Tm in the presence of F-actinPrevious studies have shown that DSC can be success-fully used for studies of the actin-induced changes in the thermal unfolding of ... capable of monitoring the thermal unfolding of Tm whenbound to actin [13,14,30], whereas CD is of limited use in the presence of actin as the signal from the six- orsevenfold molar excess of actin ... because irreversible thermal denaturation of F-actin began in this temper-ature range. In the case of smTm, the actin-induced shift in the thermal transition (i.e. the difference in Tmbetweenpeak...
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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx
... associated with the combination of factorsanalyzed in the present study (i.e. dopamine together with a-synuclein). In particular, the model suggests a potential involvement of the nuclear factorkappa ... Regardless of dopamine treatment, overexpres-sion of a-synuclein significantly affected the levels of four proteins [stathmin 1, glutathione S-transferase(GST)p, Ran 1 binding protein and C1q binding ... suggested to be linked toearly stages of PD pathogenesis [26]. Interestingly, in our model, dopamine induces an increase of the actinbundles regulator fascin 1 [27] and discordant changes of two calcium-dependent,...
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Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt
... that the PRD may be involved in regu-lating other functional domains of Mxi1 (e.g. the SID or the bHLH ⁄ LZ) and ⁄ or in the recruitment of other activ-ities. However, in some assays, the presence ... the predominanttranscript in the adult intestine and in the developingembryo, whereas Mxi1-SRb transcripts predominate in the adult liver and kidney [12]. In our initial description of Mxi1-SRa ... least in mammals, is proline and alanine rich. Hypothesizing that this PRD of Mxi1-SRa could beplaying a regulatory role or encoding novel functions,we investigated whether the presence of this...
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Tài liệu Báo cáo khoa học: Down-regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism docx
... regulation of RFC at the primary absorptive site of folic acid, i.e. the smallintestine.Expression of the RFC protein in BBM of the intestine The effect of chronic alcoholism on the level of expres-sion ... mucodepletion and an increase in intra-epithelial lymphocytes of the epithelial cells of the villus lining. There was no evidence of any haemor-rhagic mucosal lesions in the intestines of ethanol-fedrats.Estimation ... counterstaining.Haematoxylin–eosin stainingHaematoxylin–eosin staining was carried out following the routine histological method described by Kayser andBubenzer [38]. The haematoxylin–eosin staining techniqueemploys...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... differentdomains of the protein, to determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. SixGST-fused recombinant proteins containing the three distinctivedomains ... secreted into the extracellu-lar matrices of the inner ear. In the otoliths, they areboth localized in the ring-like structures. These find-ings identify for the first time proteins with these ... observed in the ventral region of the saccu-lus. (D) Hybridization signals were barely detected in the distalregion of the sacculus. (E) Hematoxylin ⁄ eosin staining of the regionshown in (B),...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... andHO-2 proteins. The intensity representing HO-1 or HO-2 protein was normalized with respect to the intensity of the b-actin signal. The ratio of each normalized value to the control value in siRNA-untreated ... short interfering RNA (siRNA) in human cells. BothHO-2 mRNA and protein are expressed in the eight human cancer celllines examined, and HO-1 expression is detectable in five of the cell lines,including ... mRNA. The intensity of the signals representing HO-1 or HO-2 mRNA was normalized with respect to the intensity of the 18S rRNA signal. The ratio of each normalized value to the control value in...
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Tài liệu Báo cáo khoa học: Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase) 6-phosphogluconolactonase docx
... because of the import-ance of this enzyme in the parasite’s intraerythrocytestage [16]. The present paper describes the effects of G6PD-6PGL silencing in P. falciparum, confirming the key role of ... nevertheless experimentally shown that this62 amino acid insertion is essential for the activity of the bifunctional enzyme [16]. In contrast, the 310amino acid protein sequence of the N-terminal ... effect of using this vector. This is the firstreport of the detection at the molecular level of the toxic effect of a DNA vector in P. falciparum.Although mechanisms of RNAi silencing in manyspecies...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... localizes in the cytoplasm of human epithelial cells of the gastrointestinal tract andliver, two central organs in the regulation of ironmetabolism [20,21]. After their synthesis in the endo-plasmic ... in iron -rich mediashowed a further increase in ROS production as deter-mined by an increase in DCFH mean fluorescenceintensity (Fig. 2B, thick line). In addition, determin-ation of acrolein ... percentage of cells in the S and G2 ⁄ M phases of the cell cycle (Fig. 3B). In a total of seven separate determinations, a statisti-cally significant decrease in the percentage of dividingHepG2...
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Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt
... inhibition of the aminopeptidasecathepsin H (Ki¼ 30 nm ). In comparison with othercystatins, cystatin F is a rather slow binding inhibitor of the cathepsins, characterized by kassvalues in the range ... Cystatins are generally rather non-selective inhibitors. An interesting feature of cystatinF is the 100-fold stronger inhibition of cathepsins F,L, and V than of cathepsin S. Cathepsin S is ... basis for the biological spe-cificity of cystatin C. Identification of leucine 9 in the N-terminal binding region as a selectivity-conferringresidue in the inhibition of mammalian cysteine pepti-dases....
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