**Báo cáo khoa học: "Analysis System of Speech Acts and Discourse Structures Using Maximum Entropy Model*" pptx**

Báo cáo khoa học: "Transparent combination of rule-based and data-driven approaches in a speech understanding architecture" pot

... medium-vocabulary command and control task.1 IntroductionAs the field of spoken language understanding be-comes more mature, a clearer picture begins toemerge of the tradeoffs between rule-based and data-driven ... withpotential users and a short videotape of a sessionwith a mock-up of the system; tight time con-strains and lack of access to users made it diffi-cult to do better than this. We transcribed and an-notated ... speech inter-actions with the system, and transcribed and an-303notated a further 424 utterances (906 words) of speech data. 75% of this was again assigned totraining and the rest saved for testing....

Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

... Yuriy Fofanov1,3 and Richard C. Willson1,71 Department of Biology and Biochemistry, University of Houston, TX, USA2 Department of Chemical and Biomolecular Engineering, University of Houston, ... screening for the presence of virus [14].Nucleic acid-based assays offer rapid and speciﬁcdetection and serotyping of dengue virus, and aregradually replacing serological and culture techniques.These ... detectinto ﬁve groups of primer pairs (Table 1 and TablesS1 and S2). Here, we report the preparation and test-ing of a mixture of 10, 18- to 22-nucleotide PCR prim-ers, each of which is at least...

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... system to ascertain the conservation of targeting mechanisms and the nature of mitochondria-targeted proteins.Mitochondrial integrity and purity were established using electronmicroscopy, and ... NADPH, and 4 mm NDMA in a 1.0 mL ﬁnalvolume. The reaction mixture was incubated at 37 °C for30 min, and the reaction was stopped by the addition of 0.1 mL of 25% zinc sulfate and 0.1 mL of saturated ... mitochondrialtargeting of CYP2E1Naresh B. V. Sepuri, Sanjay Yadav, Hindupur K. Anandatheerthavarada and Narayan G. AvadhaniDepartment of Animal Biology, School of Veterinary Medicine, University of Pennsylvania,...

Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... differentiation, and so on. Isoforms of numerousproteins have been studied and compared in this man-ner, including alternative isoforms of members of thep53 ⁄ p63 ⁄ p73 [23] and the Bcl2 families ... versions of these twoisoforms, as well as of the Mxi1-WR isoform that lacksthe SID, and shown to give rise to proteins of theexpected size expressed at similar levels (Fig. 1A). Each of these ... theeffects of the PRD are context sensitive, and coulddepend on variables including cellular milieu and pro-moter environment. It is of interest in this regard that,in our hands, Mxi1-SRa and Mxi1-SRb...

Tài liệu Báo cáo khoa học: Identiﬁcation of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... suspen-ded in 25 mL of 40% potassium hydroxide and 25 mL of methanol and refluxed for 2 h. Products were extractedwith 50 mL of hexane. The hexane layer was washed with25 mL of saturated sodium ... range of structural diversity produced by asequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini-tial origin of structural diversity of skeletons in their biosynthesis, and ... shown), none of hydroxylated triterpenes and their glycosides has been identified. The lack of understanding of triterpene metabolism in A. thalianamakes it difficult to identify the function of each...

Tài liệu Báo cáo khoa học: Thermal unfolding of smooth muscle and nonmuscle tropomyosin a-homodimers with alternatively spliced exons docx

... of actinRabbit actin was prepared by the method of Spudich and Watt [51]. Its molar concentration was determined by itsabsorbance at 290 nm using an E1% of 6.3 cm)1 and amolecular mass of ... of Tm (a and b), each containing 284residues. Smooth and skeletal muscles express differ-ent isoforms resulting from alternative splicing of thea and b genes.There are two major classes of ... nonmuscleexon 1b, and comparison of Tm5a with Tm5b allowsthe effects of exchange of exon 6 to be studied.Studies of thermal unfolding of Tm may providevaluable information on the structure of Tm both...

Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx

... addition of 100 lLof2· Kirby buffer [25] and 80 lLofH2O, and rRNA was obtained by precipitation after two phenol–chloroform extractions. Four micrograms of rRNA weretreatedwith20lL of acetic-aniline ... structure of such ahexanucleotide, where the conformation of the targetadenine had been altered, using the known structures of complexes of RTA with FMP (formycin monophosphate) and ApG (adenyl ... digital images of ethidiumbromide-stained gels, using IMAGEQUANTsoftware, and depurination was calculated by relating the amounts of the small aniline-fragment and 5.8S rRNA and expressingvalues...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... consumption and to examine the possible role played by cells and mole-cules of the immunological system in hepatic injury and repair. Understanding the interdependence betweenthe metabolism of iron and ... Igs) and Super-Signal West Femto (Perbio). Densitometry quantiﬁcation of the CD1d protein bands indicatedwas performed using a Kodak Digital Science DC40 camera and its associated software. Band ... thank L. Mayer and K. Uchida for pro-viding antibodies, A. do Vale, F. Pisarra and M.T. Sil-va for help and comments on TEM, and M. Santos and R. Hultcrantz for critical reading of the manu-script....

Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

... localization of cystatin F, its restricted and readily regulated expres-sion [18], selective and not too potent inhibition of cathepsins are all in favor of the active role of cystatinF in processes of ... lowered affinity of cyst-atin F for these enzymes. Proline in the S2site is a fea-ture of human stefin A and cystatins F, S and SN, all of which are significantly less potent inhibitors of cath-epsin ... feature of cystatinF is the 100-fold stronger inhibition of cathepsins F,L, and V than of cathepsin S. Cathepsin S is closelyrelated to other endopeptidases of the papain-likeenzyme family, and...

Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

... Research Scholar of the ThailandResearch Fund. We would like to thank Prof. Steve C.Fry, Institute of Cell and Molecular Biology, Univer-sity of Edinburgh, Edinburgh, UK, and Dr AlbertSchulte, ... Center, Nakhon Ratchasima, Thailand3 Department of Biochemistry and Center for Protein Structure and Function, Faculty of Science, Mahidol University, Bangkok, Thailand4 Max Planck Institut fu¨r ... enzymatic properties of chitinase A from Vibrio carchariae have beenstudied in detail by using combined HPLC and electrospray MS. Thisapproach allowed the separation of a and b anomers and the simultaneousmonitoring...

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