... ethanol for 2 h, washed twice with 1· NaCl ⁄ Piand stained with propidium iodide (final con-centration, 0.5 lgÆ mL)1). The cells were analyzed by flowcytommetry, using a Fusing Beckman Coulter (Fullerton,CA, ... performed immunostainingexperiments with anti-MLL1 serum using asynchro-nous HeLa cells. In contrast with our observations,they reported that MLL1 remains associated with condensed chromatins even ... progression using flow cytometryanalysis. Briefly, HeLa cells (at 60% confluence) weretransfected with MLL1-specific antisense oligonucleo-tide for 24 h, stained with propidium iodide and ana-lyzed using...