... adaptors, and cloned into the correspond-ing sites of pEFneo, using the procedure described [20].Direct DNA sequencing of the construct obtained, per-formed by the dideoxynucleotide chain-termination ... obtained by Bio-Gel P-4 purification of the included volume of the column in (A), largeO-glycans (j), obtained as the included volume of the column in (C), and small O-glycans (s), obtained as the included ... accordingto published procedures [9,16,22], with some modifica-tions. Total lysates were obtained by boiling 10 min in phosphate-buffered saline containing 0.5% SDS and 1.0%2-mercaptoethanol, and...