... induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands:s, d, 2.5 and 20 mM choline; n, m, 2.5 mM and 20 mM atropine;h, j), 2.5 mM and 20 ... min. Experimental con-ditions depended on the enzyme, and were set as follows:37 °C and pH 6.9 for LytA; 30 °C and pH 6.0 for LytC; and 30 °C and pH 6.9 for Pce. The specific activities of theenzymes ... viability and liberation of viru-lence factors. Inhibition of autolysis by excess cho-line might, in the first instance, seem to be oftherapeutic interest. However, the amount of ligandneeded, and...