Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... indicate that in vitro c-secretase cleavage
of APP substrates is inhibited by zinc and that zinc
increases the apparent molecular mass of C101-
3FLAG as determined by size exclusion chromato-
graphy. ... calculated molecular mass. Finally the
same line was used to predict the molecular mass of a
third FLAG-reactive protein between alpha and c-3FLAG
prot...
... blue and destained by 50%
methanol. The visualized protein bands on the membrane
were excised and the N-terminal amino acid sequence
of each protein was determined by automated Edman
degradation.
Cleavage ... peptide. This signal sequence is involved in
guiding the protein into the targeting and translocating
pathway by interacting with the membrane and...
... DDI- 1A and DDI-1B on AAT, CAG and
CA repeats are stronger than that of the ATT, CTG
and GT strand, which may be attributed to the TÆT
mismatch as opposed to the A A mismatch; (f) doxo-
rubincin inhibited ... on the CTG strand than the A A mispair on
the complementary CAG strand, resulting in expanded
CTG fragments that are shorter than those of the
CAG st...
... autoradiography visualized by using
GP-Storage Phosphor Screen and storm scanner and
software (Amersham Biosciences). Radioactive bands were
quantified and annealing percentage was determined by
dividing ... knuckle domains
(C-/-X-C-G-X
3
-H-X
4
-C, where / is an aromatic amino
acid and X is a variable amino acid) typical of retroviral
nucleocapsid proteins and the pres...
... AnI19R
5¢-AATTCATGAGGAGGTTTCTCTGTAACA-3¢;AnI19H
5¢-AGCTTGTTACAG-AGAAACCTCCTCATG-3¢; AnI17R
5¢-AATTCACGAGGAGGTTTCTCTGTACTA-3¢;
AnI17H 5¢-AGCTTAGTACAGAGAAACCTCCTCG
TG-3¢;AnI15R5¢-AATTCACAGGAGGTTTCTCT-GTC
TA-3¢;AnI15H5¢-AGCTTAGACAGAGAAACCTCC
TGTG-3¢. ... this statement and
suggests that the RNA binding site is still available and its
binding affinity is not altered by the presence...
... power of plasmid
A
B
C
Fig. 7. Biophysical characterization of the PRAI subdomain. (A)
Thermal denaturation of PRAI and trPRAI-His as monitored by CD
at 219 nm. Raw data and smoothed curves are shown. ... subjected to an internal duplica-
tion of the fifth (ba) unit [24]. Retention of at least
trace activity in all cases underlined the apparent
thermodynamic advant...
... coli and studied its substrate preferences, the factors
in uencing its activity and the nature of suitable metal
cofactors and inhibitors in vitro.
Materials and methods
Chemicals
The following ... washing away the chelator by
Table 1. Effect of citrate, malate and NaCl on PTOX activity in vitro.
Assays were performed in the presence of NADH, decyl-plasto...
... statistical analysis
The data were normalized in a two-step procedure. First, a
correction was applied using a factor calculated from the
intensity ratios of internal standards in the test and refer-
ence ... samples. The presence of the internal standard probes
at different locations of the array allowed quantification of
the local background and evaluation...
... in
human infections, including H. pylori strains.
2. Materials and Methods
Bacterial strains
In this study, two standard strains, that is
ATCC4350 (type strain) and ATCC43526, and 14 clinical ... Kawakami Y., Akahane T., Yamaguchi M., et al. In vitro activities
of rabeprazole, a novel proton pump inhibitor, and its thioether
derivative alone and in combination w...
... solve mathematical
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