... AshidaM, Minakata H, Oyama H, Oda K, Nishino T &Nakayama T (2004) Crystallographic and biochemicalinvestigations of kumamolisin -As, a serine-carboxyl pep-tidase with collagenase activity. ... enhance-ment of the 57-kDa band with a concomitant diminu-tion of the 38-kDa and 19-kDa bands, as analyzed bySDS ⁄ PAGE (Fig. 3A) . The 38-kDa and 19-kDa bandsdid not disappear completely after ... activesites of these enzymes contain a unique catalytic triad,Ser-Glu-Asp, in place of the canonical Ser-His-Asptriad of the classical serine peptidases. In the lattercase, the Ser and His residues act...