Báo cáo khoa học: Factors involved in the assembly of a functional molybdopyranopterin center in recombinant Comamonas acidovorans xanthine dehydrogenase pot
... with a
calculated average molecular mass of 57 752 Da and the
a- subunit contains 808 amino acids with a calculated average
molecular mass of 87 392 Da. The total calculated average
molecular mass ... a functional Mo catalytic
center. The functionality is estimated as a ratio of change in
the absorbance at 450 nm after anaerobic reduction of the
enzyme with 1 m...
... of
immunoreactivity in the latter strain was due to the inability
of the truncated protein to insert into and be stable in the
inner mitochondrial membrane or lack of detection of the
truncated protein ... c
1
and a disappearance of the intermediate form
of the Rieske protein. At the same time, the levels of
subunits 7, 8 and 9 significantly decreased in...
... time-dependent increase of the mRNA abun-
dance in A7 r5 cells incubated at 1% oxygen for P4ha1 and
P4ha2, starting around 4 h of hypoxia, the induction of
P4ha2 mRNA being stronger than that of P4ha1 (Fig. ... as the relative mRNA/b-actin mRNA ratio. The
mRNA/b-actin mRNA ratio of the time standards (pools)
cDNA was set to 1.0 (i.e. normoxia, 21% oxygen). Data
are ther...
... execute commands.
The main functions of ATLAS are the following :
- Editing and updating of indexing charts : compi-
lation of an external form of the chart, and
modification of the internal form ... may be represented by associating
questions to each node and the possible answers to
the arcs coming from a node ; the leaves of the tree
bear the name of...
... were
used as primary antisera (Table 3). Secondary antibodies
(anti-rat IgG, anti-guinea pig IgG and anti-rabbit IgG; all
raised in goat and conjugated to alkaline-phosphatase;
Sigma, Saint Louis, ... France
Introduction
Modification of the chirality of a single aminoacyl resi-
due within a peptide chain is a subtle and intriguing
mechanism that remains poorly known to date, and...
... The role of the ESSS protein in the assembly of a functional and stable
mammalian mitochondrial complex I (NADH-ubiquinone
oxidoreductase)
Prasanth Potluri, Nagendra Yadava and Immo ... subunits of the integral membrane-
subcomplex also assemble via the formation of distinct
and i dentifiable assembly intermediates.
The mutants promise to be valuable tools in t...
... adaptations in the eye in the human lineage.
In addition to the evolution of cN and cS crystal-
lins, even more specialization in the c-crystallins has
occurred with the loss of the N-terminal ... use of animals complied with the ARVO Statement
for the Use of Animals in Ophthalmic and Vision Research
and the Intramural Animal Care and Use program of the...
... and arabinogalactan [6,7]. ABNs attack the
glycosidic bonds of the a- 1,5-l-arabinan backbone,
releasing a mixture of arabinooligosaccharides and l-
arabinose [4]. These types of enzyme have attracted
much ... and
de-branched arabinans, and heteropolysaccharides
such as arabinoxylans and arabinogalactans. Arabinan
is composed of a- 1,5-linked l-arabinofuranosyl units,
some of...
... designated HeLaTR/4-1BB and HeaLaTR/
TRAF1, respectively. Primers for amplifying the 4-1BB and
TRAF1 cDNAs were: 5¢-GAATTCAAGCTTATGGGA
AACAGCTGTTACAACATA-3¢ and 5¢-GAATTCAAG
CTTCACAGTTCACATCCTCCTTCTTCT-3¢ ... hTRa1
cDNA were: 5¢-CCCGGGAAGCTTCGGACCATGG
AACAGAAGCCAAGCAAGGTG-3¢ and 5¢-CCCGGG
GTCGACGACTTCCTGATCCTCAAAGACCTC-3¢ .In
order to overexpress 4-1BB and TRAF1 in the HeLaTR
cells, t...