Báo cáo khoa học: Kinetic mechanism for p38 MAP kinase a A partial rapid-equilibrium random-order ternary-complex mechanism for the phosphorylation of a protein substrate potx
Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx
... that of 4 by the presence of a 2-acetamide group adjacent to the p-nitro-phenyl group. This was the same in the case of a comparison of 1 and 3. Furthermore, the enzyme also catalysed a transglycosylation ... respectively.Assay of endo-b-galactosidase activity by HPLC The standard assay was carried out as follows. A reactionmixture (500 lL) containing an appropriate substrate andendo-b-galactosidase in ... enzyme was1.7-times higher than that for keratan sulfate as shown inTable 3, indicating that it is very useful as a substrate instead of keratan sulfate for analytical use in the endo-b-galactosidase...
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Báo cáo khoa học: Kinetic mechanism for p38 MAP kinase a A partial rapid-equilibrium random-order ternary-complex mechanism for the phosphorylation of a protein substrate potx
... mechanism for p38 MAP kinase a Kinetic mechanism for p38 MAP kinase a A partial rapid-equilibrium random-order ternary-complex mechanism for the phosphorylation of a protein substrate Anna E. Szafranska1and ... three-tieredMAPK modules, each of which consists of a MAP kinase (MAPK), a MAPK kinase (MAPKK) and a MAPKK kinase (MAPKKK). MAPK modules oper-ate in numerous biological settings where, throughlargely ... Titration of AMP-PCP into a mixture of activated p38 MAPKa (75 lM) and ATF2D115 (97 lM); (lower) integrated enthalpy change for each injection. Kinetic mechanism for p38 MAP kinase a A. E. Szafranska...
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Tài liệu Báo cáo khoa học: Kinetic basis for linking the first two enzymes of chlorophyll biosynthesis doc
... quenched-flow analysis reveals that ChlH dramat-ically accelerates the formation and breakdown of an intermediate in the catalytic cycle of ChlM. In light of the profound effect that ChlH has on the methyltransferase ... a higheravailable concentration of ChlM to bind other mole-cular species in the reaction.ChlH appears to enhance catalysis by accelerating the formation and decay of a catalytic intermediate.This ... pathways. However, the relative catalytic rates of magnesium chelatase and MgP methyltransferase maydictate that there is very little free MgP available for signalling. We have estimated that...
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Báo cáo khóa học: ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK docx
... cells.As noted above, both the p38 and ERK MAPKcascades are variously implicated in the activation of NF-jB. However, as many of these studies rely on transfectionanalysis of reporter plasmids, ... involve-ment of the p38 mitogen activated protein kinase (MAPK)and the extracellular signal-regulated kinases (ERK) in the activation or potentiation of NF-jB-dependent transcrip-tion [5]. Thus, the selective ... protein kinase (MAPK)cascades in the activation and transactivation of NF-jB.We show here that SB203580, a selective inhibitor of the p38 MAPK, and PD098059 and UO126, both selectiveinhibitors of...
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Báo cáo khoa học: Kinetic deuterium isotope effects for 7-alkoxycoumarin O-dealkylation reactions catalyzed by human cytochromes P450 and in liver microsomes Rate-limiting C-H bond breaking in cytochrome P450 1A2 substrate oxidation pdf
... coumarin, the value was high for P450 1A2 (9.5); with 7-OMe coumarin the value for P450 1A2 was 10.6 and for P450 2E1 was 7.7. The value of these measurements lies in their comparisonwith the values ... microsomes.Results and discussionIntramolecular kinetic isotope effects The intramolecular kinetic isotope effects measure the comparative rates for cleavage of a C-H bond and a C-D bond at a single carbon ... independent of what the rate-limiting steps are for these two enzymes (andP450 2A6 [19]), if similar mechanisms are operative.At least two factors can perturb these values so thatthey are not...
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Báo cáo khoa học: Kinetic hybrid models composed of mechanistic and simplified enzymatic rate laws – a promising method for speeding up the kinetic modelling of complex metabolic networks pptx
... designates the type of load parameter varied and the range of variation relative to the normalvalue of the reference state. The last four columns show the percentage of the total variation range of the ... 2008 The Authors Journal compilation ª 2008 FEBS for the construction of the Jacobian matrix used for the analysis of stability. Enzymatic rate laws and otherdetails of the full kinetic model are ... increased value of kATPaseas compared to the value k0ATPase¼ 1:6hÀ1. At values of kATPaseexceeding seven-fold of its normal value,no stationary states can be found; that is,kmaxATPase¼...
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Báo cáo khoa học: "Veterinary decision making in relation to metritis - a qualitative approach to understand the background for variation and bias in veterinary medical records"
... collection of the data in and of itself as the basis for taking relevant actionat the farm. They may skip the process of systematic anal-ysis of data and give advice based on their immediate eval-uation ... or her evaluation of the localcontext. That is, treatment data as an indicator of a certaindisease manifestation may only be valid within the herd.When veterinarians used standard treatment ... tool' related to each individual treatment decisionrather than being part of a collaborative data collection. For example, they could add rectal temperature and otherparameters into the...
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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf
... recombinantenzymes free of albumin and any other AAAs. It wasfound that there was no fundamental difference in the mechanisms of inhibition and activation for either the AAA or esterase activities ... 7.0 at 25 °C. The effect of each ligand on related pairs of substrates(neutral substrates o-NAC and o-NPA, and positivelycharged substrates ATMA and ASCh) was compared. Toavoid complications ... hydrolases that catalyse the deacylation of N-acyl arylamines [20,21]. SeveralAAAs have been identified in mammalian tissues[22,23]. Certain acryl acylamidases are identical tocarboxylesterases...
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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... mM Mes (pH 7) and substrate (5¢-G2CCCUCUAAAAA-3¢)at50°C[6].c Substrate- cleavage reaction (exogenous guanosine-mediated) of the substrate (5¢-CUUAAAAA-3¢) using the Anabaena ribozyme(L-8 ... dissociate, the similar order of magnitude suggests that a non-trivial fraction of the substrate will dissociate before the substrate- cleavagereaction occurs. The ‘catalytic power’ of an RNA-cleaving ... is4.1Æmin)1. Although the true rate constant for the actual chemical step is being masked, probably by a local conformational change that occurs after substrate binding and before the actual chemical step,...
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Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt
... identify the extracellular ligands of the RPTPs in the neuromuscular system. For example, it is knownthat PTPd and an isoform of LAR can interacthomophilically [39] and that LAR can also bindheterophilically ... black dots, protease cleavage sites. (B) SDS ⁄ PAGEseparation of FN3d–AP purified from conditioned media using anti-placental alkaline phosphatase (PLAP) agarose. (C) SDS ⁄ PAGE and silverstain ... semipermeabilize cells for intracellular staining, cellswere incubated in 4% paraformaldehyde for 30 min at 4 °Cin the first instance and then stained as described above.Confocal microscopy and imagingE10...
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