Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebrafish genome by subfunctionalization of tissue-specific expression doc
... al. Duplicated crabp1 genes in zebrafish
FEBS Journal 272 (2005) 35 61 35 71 ª 2005 FEBS 35 71
Retention of the duplicated cellular retinoic acid-binding
protein 1 genes (crabp1a and crabp1b) in the ... accepted 16 May 2005)
doi :10 .11 11/ j .17 42-4658.2005.04775.x
The cellular retinoic acid-binding protein type I (CRABPI) is encoded by...
... positioned, the tricyclic F
420
ring is sand-
wiched between the isoalloxazine ring of FMN and the
segment between His1 51 and Pro153 of the switch
loop, whereby the imidazole group of His1 51 interacts
with ... activity. The amino acids
involved in FMN binding are highlighted in yellow,
and those involved in iron coordination are highlighted
in red. The am...
... showed
that a linear sugar molecule was bound (Fig. 3D).
Again, the phosphate group interacts with His 11,
Arg 113 , Arg137¢ and Arg1 41 . The other end of the
substrate points into a deep pocket in the enzyme.
Moving ... 24.7 1. 9 (2.00 1. 90) 30.0–2 .1 (2. 21 2 .10 ) 29.4–2 .15 (2.27–2 .15 )
Number of reflections measured 14 1 283 455 063 2 91 2 91 108 089 13 1 784
Numb...
... subsite )1
more deeply than that of P2. This may be caused by
Fig. 5. Stereoview of the catalytic site of D356N ⁄ P2. The binding of P2 is shown in green, and the binding of G6 and Phe 313 in
D356N ... side chain
depending on the structure of the substrate to form
favorable interactions with the substrate. In the bind-
ing of G6, Phe 313 mainly interac...
... rapidly and the
propensity to err rapidly increases after 16 hours of sustained
wakefulness, a finding reflected in the twofold increase in
interns’ attentional failures after they had been working ... intensive
care unit during the studies noted that interns in the
intervention schedule “often knew very little about the
patients who had been admitted the night before”...
... lLofthe
supernatant after addition of [
3
H]cAMP and bovine
adrenal binding protein and incubation for 2 h at 4 °C.
Standards containing nonlabeled cAMP were assayed in
the same manner. The incubates ... in the human
MC1 receptor and these are over-represented in Northern
European redheads and in individuals with pale skin [17 ,18 ].
These variants are a risk factor,...
... strategy
The aim of this study was to establish a rapid and reli-
able method for the detection of protein protein inter-
actions using yeast G -protein signaling. In our system,
protein protein interactions ... BZG 211 8 (ZZ
mem
), BFG 211 8 (Gc
cyto
–Fc)
and BZFG 211 8 (ZZ
mem
⁄ Gc
cyto
–Fc) (Table 1) . Expres-
sion of ZZ
mem
proteins in BZG 211 8 (lane 2) and
BZFG...
... Most of them were identified
due to the presence of their genes in the close vicinity
of cognate protein kinase genes [12 14 ]. Another
approach which could make the identification of sub-
strates of ... (10 0 %) and compared with the activity of mutated enzyme StkPK42R
(lane 2) and StkP in the presence 0 .1 m
M,1mM and 10 mM of staurosporine (lanes 3,...