... conformationaltransitions linked to Na+bindingRaimondo De Cristofaro1, Andrea Carotti2, Sepideh Akhavan3,*, Roberta Palla3, Flora Peyvandi3,Cosimo Altomare2and Pier Mannuccio Mannucci31 ... enzymaticexperiments, an increase of % 0.5 pK units of theHis57 in DK9 mutant was also calculated analyzing theNAPAP data set (Table 1C). In fact, His57 undergoesTable 1. Best-fit pK a values of ... overall stability of the molecule, weakens the A B interchain interactions, and allostericallyperturbs the geometry and protonation state of catalytic residues of theenzyme.Abbreviations a- NAPAP,...