... hydrophobicinteractions, such as F(109:Y) and L(70:Y), are alsoconserved, and may align with F(100:C), F(102:G) and L(59:C), L(61:G), respectively. On the other hand,R(66:Y), R(75:Y) and K(102:Y) do not align ... plateau, rapidlyextracted with phenol, and the aminoacylated tRNA wascollected by ethanol precipitation at pH 4.8.Alternatively, the procedure described by Wolfson and Uhlenbeck [28] to detect the ... [14C]-canavanine) and dried.Aliquots were spotted onto the discs which were then washedwith two changes of 5% trichloroacetic acid and once withethanol (10 min each), before being dried and quantified by scintillation...