... colorimetric assay for uricase activity and a screenfor mutantBacillus subtilis uricasegenes following StEP mutagenesisSu-Hua Huang and Tung-Kung WuDepartment of Biological Science and Technology, ... ofmutant uricase. Then, they were treated at 60 °Cfor1hforcell lysis and release of the protein. Following the addition ofsubstrate, the plate was incubated for 10 min at 37 °C, abright purple ... 521more efficient than conventional methods (Fig. 1), whichtake at least 4 days and include the following steps: 2 mLLB broth/ampicillin addition, purification of plasmid DNA, digestion, electrophoresis,...