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Báo cáo khoa học: Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy pot

Báo cáo khoa học: Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy pot

Báo cáo khoa học: Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy pot

... spectra of DNA photolyase. (A, A¢) Photore-duction of DNA photolyase (two different batches of protein). (B)Photoreduction of [U-15N] -DNA photolyase. (C) Photoreduction of DNA photolyase ... difference spectra of DNA photolyase and DNA. (A)Oligo-(dT)18 DNA photodamage with UV radiation in the absence of photolyase. (B) Photoreactivation of DNA photolyase followed by DNA photorepair ... Journal 272 (2005) 1855–1866 ª 2005 FEBS Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy Erik Schleicher1,*, Benedikt Heßling2, Viktoria...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx

Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx

... Activation of the acetyl phosphate synthesis activity of E. coli Pta by different concentrations of pyruvate and PEP. Results are presented as percentage of activity in the presence of PEP or ... primer, 100 ng of DNA template, 5 lL of 5· GoTaq DNA polymerase buffer, and 0.6 U of GoTaq DNA polymerase (Promega, Madison, WI, USA).The amplification protocol was as follows: one cycle of 2 min at ... the biochemical character-ization of E. coli Pta and set out to investigate thefunction of its N-terminal domain by the construc-tion and analysis of three E. coli Ptas with deletionsfrom the...
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Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis potx

Báo cáo khoa học: Limited proteolysis of Escherichia coli cytidine 5¢-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis potx

... only treatment of CTPS with trypsin produced a limited number of cleavagefragments over the course of 1 h, of which the formation of some fragments was suppressed in the presence of ATP and/or ... hydrolysis of glutamine, but had a value of kcatthatwashalfofthatobserved for wild-type CTPS in the absence of GTP. Whenthe concentration of GTP was increased to 1 mM,thevalue of kactwas ... FEBS 2003Limited proteolysis of Escherichia coli cytidine 5¢-triphosphatesynthase. Identification of residues required for CTP formationand GTP-dependent activation of glutamine hydrolysisDave...
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Báo cáo khoa học: Functional interaction of Escherichia coli heat-labile enterotoxin with blood group A-active glycoconjugates from differentiated HT29 cells docx

Báo cáo khoa học: Functional interaction of Escherichia coli heat-labile enterotoxin with blood group A-active glycoconjugates from differentiated HT29 cells docx

... HT29-5F7. The presence of non-GM1 recep-tors of Escherichia coli heat-labile enterotoxin (LT-I) on both types of dif-ferentiated HT29 cells was indicated by the inability of cholera toxin Bsubunit ... K-A(1994) Comparison of the glycolipid-binding specificities of cholera toxin and porcine Escherichia coli heat-labileenterotoxin: identification of a receptor-active non-gang-lioside glycolipid for the ... effect of 125I-labelled LTbinding by H. pomatia lectin was also determined by pre-incubation of cells with lectin, as indicated for B subunits of toxins.Toxin-stimulated accumulation of intracellularcyclic...
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Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx

Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx

... phos-photransferase system (PTS) in Escherichia coli is one of the best studied biochemical networks from theo-retical and experimental points of view, and hasKeywords Escherichia coli; model verification; ... modeling of thecentral carbon metabolism of Escherichia coli. Biotech-nol Bioeng 79, 53–73.8 Mahadevan R, Edwards JS & Doyle FJ (2002)Dynamic flux balance analysis of diauxic growth in Escherichia ... signaltransduction models in Escherichia coli. Bioinformatics24, 2044–2050.14 Kremling A, Bettenbrock K & Gilles ED (2007) Analy-sis of global control of Escherichia coli carbohydrateuptake....
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Báo cáo khoa học: Adventitious reactions of alkene monooxygenase reveal common reaction pathways and component interactions among bacterial hydrocarbon oxygenases ppt

Báo cáo khoa học: Adventitious reactions of alkene monooxygenase reveal common reaction pathways and component interactions among bacterial hydrocarbon oxygenases ppt

... was investigated by monitoring the growth of flask cultures of R. rhodochrous B-276 in the presence of a range of alkynes (Fig. 1). A fivefold molar excessAdventitious reactions of alkene monooxygenase ... regeneration of NADH. In thecontext of a whole-cell biocatalyst that would bypassproblems with coenzyme regeneration, we began thepresent study of AMO by investigating the effect of alkynes ... activity was measured as a function of the time between the start of the reaction (addition of NADH) and removal of the alkyne by flushing withnitrogen. First-order decay of enzyme activity wasobserved,...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... BamHI–EcoRIsites of pGST–pHis, a cDNA fragment encoding aminoacids 1–165 of calpain 7 (calpain 7MIT) was amplified by using a pair of primers (forward, 5¢-GAGAGATCTEnhancement of calpain 7 autolysis by IST1 ... were proba-bly derived by limited digestion with other cellular pro-teases, and not by autolysis of mGFP–calpain 7.Enhancement of autolysis of mGFP–calpain 7–Strep by GST–MIM in vitroAs we ... analyzed by WB with mAb against GFPto detect proteolysed fragments of mGFP–calpain 7–Strep. Bands of full-length WT and C290S are indicated by the arrow, and those of 30 K are indicated by the...
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Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc

Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc

... carbonyl group of Asn7 of Tat(1–9) and theside chain of Lys554 of DP IV and finally, between thecarbonyl group of Ile8 of the peptide interacting with theTyr752 hydroxyl group of the enzyme. ... andAsp709 as well as Asp739 of DP IV, between the C-terminalGlu9 of Tat(1–9) and the side chains of Arg560 and Lys554 of DP IV. Furthermore, the side chain of Asp5 of Tat(1–9) wasalso able to ... the side chain of Lys554 of DP IV.Additionally, hydrogen bonds were formed between thebackbone carbonyl group of Val4 of the peptide and thebackbone amide hydrogen of Ala743 of the enzyme,...
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Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot

Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot

... conformation of ETNF-6mAbSequencing of the cDNA of the variable region of ETNF-6 mAb was performed, and this was followed by molecular modeling of its three-dimensional struc-ture. The heavy chain of ... the Ser5-Arg6 bond of TNF-a at the first step, resulting in the generation of a fragment of  17 kDa. This fragment was digested to a smaller molecule of 15 kDa by scission of the Gln21-Ala22 ... reaction time of 47.2 h, the amount of TP41-1 at aretention time of 11 min decreased, because of frag-mentation. The fragment was observed at a retentiontime of 13 min (indicated by an arrow)...
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Báo cáo khoa học: Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase pot

Báo cáo khoa học: Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase pot

... inhibitors. Binding of 6PG and5PR poorly affects the dissociation constant of the coenzymes, whereasbinding of 4PE decreases the dissociation constant of the coenzymes by two orders of magnitude. ... enzyme has been obtained by studying thebinding of aPyADP in the presence of 6PG [23], and by observing that 6PG enhances the decarboxylation of 3-keto-2-deoxy 6PG, an analogue of the putativeintermediate ... is present. In the case of 4PE, the Kdvalue of the inhibitor is close to the Kdvalue of thesubstrate, but it decreases by two orders of magnitudein the presence of both oxidized and reduced...
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