... Activation of the acetyl phosphate synthesis activity of E. coli Pta by different concentrations of pyruvate and PEP. Results are presented as percentage of activity in the presence of PEP or ... primer, 100 ng of DNA template, 5 lL of 5· GoTaq DNA polymerase buffer, and 0.6 U of GoTaq DNA polymerase (Promega, Madison, WI, USA).The amplification protocol was as follows: one cycle of 2 min at ... the biochemical character-ization of E. coli Pta and set out to investigate thefunction of its N-terminal domain by the construc-tion and analysis of three E. coli Ptas with deletionsfrom the...