... FEBS
Characterization of the Drosophila Methoprene -tolerant
gene product
Juvenile hormone binding and ligand-dependent gene regulation
Ken Miura, Masahito Oda, Sumiko Makita and Yasuo Chinzei
Department of Medical ... activator of gene
regulation [45]. Contrary to this, MET exists in the
nucleus even in the absence of ligand. Upon ligand
bindi...
... 14-3-3s.
Results
The C-terminus of isoform 1 of the PM H
+
-ATPase
of A. thaliana (AHA1) interacts with the first 88 amino
acids of PPI1 [22], indicating that the PM H
+
-ATPase
binding site of PPI1 is ... stress,
and their binding to the H
+
-ATPase is suggested by
the parallel increase of the number of FC -binding
sites [18–20]. As to auxin, a soluble auxin...
... Presumably, their homologues SNO2,
SNO3, SNZ2 and SNZ3 complement the defect. The
relationship of all of these genes remains to be clarified.
Expression and purification of Sno1p and Snz1p
In light of the ... and
generated by the product of gene pdxA, is not involved,
compounds related to it structurally could be the coupling
partner of the ketopentose in yea...
... out in triplicate, and the error bars indicate the
standard deviation.
Fig. 8. Effects of mutations in the Ets- and GATA -binding sites and the
E-box of the mouse a3 integrin gene on promoter activity. ... structures of the mouse a3integrin
subunit gene including the exon/intron organization and the
alternative exon usage for the generation of variants o...
... Cleavage of the C9–C10
double bond (a) results in products 1 and 2 from I, products 1 and
3 from II, and products 3 and 4 from III. The cleavage of (b) leads
to product 4 from II, and product ... preference of OsCCD1 for the
C5¢–C6¢ and C7¢–C8¢ double bonds depends on the
nature of the substrate.
The cleavage of a sole double bond in the cyclic moi-
ety...
... kDa, and, unexpectedly, another specific and
even stronger band appeared higher up in the gel
(Fig. 1B, lane 6). A subsequent incubation of the gel
in DPI resulted in inhibition of the lower band ... arrows).
Owing to the horizontal smear, however, the presence
of the B14 subunit in the lower band cannot be ascer-
tained at this point. A cross-reaction of the antibod...
... footprinting of the 5¢
upstream region of the a-F1-ATPase gene and found that
the combination of GAF and Adf-1 binding sites is present
both in o ther Drosophila species (D. yakuba and D. pseu-
doobscura)andinAnopheles ... several
transcription factors and coactivators that regulate the
expression of genes playing key r oles in the biogenesis of
the OXPHOS...
... trans-
ferase that synthesizes (p)ppGpp. Production of this
alarmone results in a down -regulation of stable RNA
synthesis (rRNA and tRNA) and up -regulation of the
synthesis of mRNAs encoding enzymes ... 6E).
From these results it can be concluded that tRNAs
were bound in classical states with the tRNA
Met
f
bound
in the P-sites of the 30S and 50S subunits and th...
... methanol [35]. The
random coil value of Pro was used for the
different prolinoamino acids. Therefore, the
CSDs of P
3
Leu10 depend primarily on the
chemical modification of the pyrrolidine ring
and cannot ... and
EC
50
values for the cAMP and IPs pathways, and are
reported in Table 1 [20,28]. The affinity of the trans
analogue [P
t
3
Leu10]SP is similar to that o...
... composed
of multiple species with the N-termini of Asn43, Val46
and Ile56, and that at a large dose (lane 6) consisted
of a single species with the N-terminus of Ile56
(Table 1). The N-terminus of the ... propeptide
of subtilisin adopts an arranged structure only in the
presence of the mature form of the protease [23].
Whether or not a similar mechanism is re...