... ligand is involved in the initial homing of leuko-cytes to sites of in ammation [73,74]. The binding of PSGL-1 to P-selectin is largely due to two PTMs,namely an O-glycan that contains tetrasaccharidesialyl-LewisX, ... istaxing, as the biosynthesis of glycoproteins is not tem-plate driven. This results in the formation of so-called‘glycoforms’ [11,12], proteins with the same peptidebackbone that differ in the nature ... (lacZ)reporter protein. In all cases, the proteins maintainednative function as well as being endowed withadditional lectin-binding properties. The two methods of modification, although employing different...