Báo cáo khoa học: Interaction of an 40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf

... 2004 Interaction of an  40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver Sujata ... present in normal and regenerating rat liver with the )148 to )124 region of c-jun In order to establish w hether the factors p resent in rRNE-dbind to the )148 to )124 region of c-jun and form a complexdifferent ... inducible factorinteracts with R LjunRP bound to the )148 to )124 region, and further enhances c-jun transcription in proliferating liver. Most of the studies to gain an insight into the transcrip-tional...

Báo cáo khoa học: Shewasin A, an active pepsin homolog from the bacterium Shewanella amazonensis pptx

... in the shewasin A amino acid sequence. The Asp of the active site motif from the N-terminal domain (marked with an aster-isk) was mutated to an Ala to generate the active site mutantshewasin ... trans-formed into E. coli BL21(DE3). The method of recombi-nant protein expression was optimized to maximize the yield of protein in soluble form, and the resulting condi-tions were used in ... reﬂecting that of the bacterialcytoplasm, i.e. close to neutrality. The presence in shewasin A of an Ala just downstream from the Asp-Thr ⁄ Ser-Gly motif of the C-terminal domain (in the sequence...

Báo cáo khoa học: FH8 – a small EF-hand protein from Fasciola hepatica docx

... (2009) An integrated transcriptomicand proteomic analysis of the secretome of the helminthpathogen, Fasciola hepatica: proteins associated with invasion and infection of the mammalian host. ... array of proteinsinto the host, and transcriptomic and proteomicapproaches, during the different life stages of the para-site, have been applied to investigate the importance of these proteins ... monitored for 3 min at severaltemperatures, ranging from 20 to 98 ° C. At each datapoint, the sample temperature was allowed to equilibratefor 2 min. The thermal unfolding of the protein was totallyreversible,...

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... revealed the structural and func-tional determinants of the protein protein interaction between v-KIND and MAP2 (i.e. the MAP2-bindingcore module in v-KIND and the v-KIND-binding coremodule in MAP2) ... v-KIND KIND2 binding resides withinresidues 702–744 of MAP2.Residues 456–487 in the KIND2 domain of v-KINDcontain the core MAP2 binding module To identify the core MAP2-binding site within the KIND2 ... andbranching.Results The KIND2 domain of v-KIND has a unique ability to localize to dendrites via MAP2 binding,which is absent in the KINDs from otherKIND-containing proteins To examine the...

Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt

... wasunable to use ammonia as a substrate and was distinct from alanine dehydrogenase [18] and N-methylalaninedehydrogenase found by Lin & Wagner [17]. Lysineand ornithine were inert as an amine ... concentrations of methylamine intersected to the left of the 1 ⁄ v axis. Similar results were obtained with various concentrations of NADPH and ﬁxed con-centrations of pyruvate. These indicate ... noncompetitiveinhibitor with regard to NADP+and N-methyl-l-alan-ine. Methylamine behaved as a mixed-type inhibitor with respect to NADP+and N -methyl-l-alanine. Theseresults indicate that...

Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

... analyzed the effect of HSP25 on the polymerization of intact actin andaggregation of heated actin. Interaction of HSP25 with intact actin In the ﬁrst series of experiments we analyzed the effect of HSP25 ... After staining, the gels were scannedand evaluated by the ONEDSCANprogram. The intensity of the band of unhydrolyzed actin was plotted against the time of incubation.Characterization of actin preparation ... starting the investigation of the HSP25–actin inter-action it was desirable to characterize the properties of intactand heated actin. We were mainly interested in the irrever-sible changes in the...

Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... shown to negatively affect the binding of ATP to GlnK [12]. Therefore, we investigated the binding of TnrA to GlnK in the presence of differentmixtures of Mg2+or Mn2+ with the effector moleculesATP ... as an analyte in SPR analysis.AMP and glutamine are known to be the most effec-tive inhibitors of GS [9]. Figure 5B shows the effect of the feedback inhibitors AMP and glutamine on GSbinding ... resolve the inhibitoryeffect of ATP on the GlnK–TnrA interaction in the absence of divalent cations more clearly, ATP wastitrated to the binding assays in the absence or pres-ence of 2-oxolguatarate....

Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt

... PP4(C), and PP6(C)) andanalysis of the interaction of PP2A(C) with a4 protein. Protein Expr Purif 31, 19–33.26 Prickett TD & Brautigan DL (2004) Overlapping bind-ing sites in protein phosphatase ... cDNAs) and PP6c (twocDNAs). Initial mapping of the region of PP2Acinvolved in TIPRL binding was obtained from the cDNAs that showed positive interaction with TIPRL. The extension of these cDNAs ... reg-ulation of type 2A phosphatases and mTOR signaling. In this study, starting from yeast two-hybrid analy-ses, we identiﬁed the interaction of TIPRL with the C-terminal region of the catalytic...

Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot

... Importantly, signiﬁcantamounts of CcCac1L and CcLim15 were localizedwithin the nuclei from the leptotene/zygotene stage to the pachytene stage. To examine the interaction between CcCac1L andCcLim15 ... [16], the sumoylation pro-tein Ubc9 [17] and the DNA synthesis-related factor proliferating cell nuclear antigen (PCNA) [18] havebeen reported to interact with Lim15/Dmc1. Theseproteins seem to ... meiosis-speciﬁcrecombinase Lim15/Dmc1 interacts with the homologue of the largestsubunit of chromatin assembly factor 1 (CAF-1) in the basidiomyceteCoprinopsis cinerea (Coprinus cinereus). Using C. cinerea...

Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc

... of the interaction of viral fusion proteins with membranes and the mechan-ism by which these proteins accelerate the formation of membrane fusion intermediates are poorly under-stood [2]. In ... as an internal fusionpeptide. In this study we have analysed the interactions of an N-terminal and an internal peptide sequence of the E2structural protein of HGV ⁄ GBV-C with model mem-branes, ... containing amino acids 98–110 of the ﬂavivirus E protein) may serve as an internalfusion peptide, as it is directly involved in interactions with target membranes during the initial stages of membrane...

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Báo cáo khoa học: Interaction of an  40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf

Báo cáo khoa học: Interaction of an 40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf