Báo cáo khoa học: Classiﬁcation of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains pdf

... o f Lon proteases, LonA and LonB, based on the consensus sequences in the active sites of their proteolytic domains. These differencesstrictly associate with the specific characteristics of their AAA+modules, ... Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains Tatyana V. Rotanova1, Edward E. Melnikov1, ... in the N domain, one in each of the a/b and a domains of the AAA+module, and three in the P domain. The majority of LonA proteases contain between 1 and 11 Cys residues,although  2% of these...

Báo cáo khoa học: "Classiﬁcation of Feedback Expressions in Multimodal Data" pdf

... three sets of experi-ments, first looking at how prosodic features con-tribute to the classification, then testing whether the use of head gesture information improved the accuracy of the classifier, ... to other corpora, both spoken and written, and a reason why we decided to use the DanPASS videos in spite of the fact that the gesture behaviour is relatively limited given the fact that the ... other. Furthermore, the restricted contextsin which feedback expressions occur in these di-alogues allow for a very fine-grained analysis of the relation of these expressions with prosody and gestures....

Báo cáo khoa học: "Classiﬁcation of Semantic Relationships between Nominals Using Pattern Clusters" pdf

... scholarships for students and professionals ’, we use the symmetric pattern ‘X and Y’ to add the word scholarships to the group of loans and to add the word professionals to the group of students. We do ... share at leastS percent of their patterns, and all of their core patterns, with C1.ii. Add all patterns of C2to C1, setting allshared patterns as core and all others asunconﬁrmed.iii. ... including those in agiven classiﬁcation problem, will be represented bysome of the discovered clusters. We then use the training set to label some of the clusters, and the la-beled clusters to...

Báo cáo khoa học: Classiﬁcation of the short-chain dehydrogenase ⁄reductase superfamily using hidden Markov models potx

... and 52extended (68% and 17%, respectively). In total, thesecover about 27 900 proteins, and surprisingly, giventhat the majority of the families are classical, 36% of the proteins are of the ... each of the candidate sequences was compared with all of the othercandidates using fasta [23]. We tested clustering at variouslevels, and found that an initial clustering at the 40% level and ... toachieve the desired speciﬁcity. If two clusters were equal,one of the HMMs was selected, and if one cluster was asubset of another cluster, the HMM of the larger clusterwas selected. If there...

Tài liệu Báo cáo khoa học: "A Method for Correcting Errors in Speech Recognition Using the Statistical Features of Character Co-occurrence" pptx

... arbitrary candidates, when one of their Error-Parts includes the other, and their frequencies are the same value, the candidate whose Error-Part includes the other is accepted. Condition of Inclusion-2: ... improving the understanding of the recognition results. On the other hand, there were four level-down cases. Three of these cases were caused by the misdetection of errors in the SSC procedure. The ... In all other cases, go to the step for a middle. Here, P1 and P2 denote the start and end positions of an error-block, and L denotes the length of the input string. Step 2: Take the string...

Báo cáo khoa học: Heavy metal ions inhibit molybdoenzyme activity by binding to the dithiolene moiety of molybdopterin in Escherichia coli pdf

... form of the molybdenum cofactor. The ability of various metals tobind tightly to sulfur-rich sites raised the question of whether other metalions could be inserted in place of molybdenum at the ... to the molybdate- or tungstate-substituted form of MPT, and not to other metal-substituted forms of MPT. The chemical and physicalsimilarities of tungstate and molybdate are a result of their ... the insertion of metalions into MPT beyond the reaction by the MogA and MoeA proteins. The insertion of copper, cadmium and arsenite is independent of the MogA and MoeA pro-teins. Even if the...

Báo cáo khoa học: a-1 Antitrypsin binds preprohepcidin intracellularly and prohepcidin in the serum pdf

... inhepatocytes and macrophages, and reduces the ironlevel in the plasma. The known signals for the induc-tion of hepcidin synthesis are the elevation of the plasma iron level, inflammation and bacterial ... interactionassays. The cDNA coding the 84-AA preprohepcidin wasamplified and cloned into pBT of the kit with the restrictionenzymes BamHI and XhoI. Tables S1 and S2 show the con-structs and primer ... possiblethat the antibody in the ELISA kit is not always ableto react with the prohormone because it is coveredby the protease inhibitor. In the case of inflammation, the blood level of the active...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... poly G and poly U RNA in vitro [8]. On the other hand, Hume et al. [15] suggested that EWSbinds to the proximal-element DNA of the macro-phage-speciﬁc promoter of the CSF-1 receptor gene. The ... determinewhether RGG3 of EWS modulates the formation orunwinding of Htelo G-quadruplex DNA. To determinewhether the RGG3 binding affected the stability of the G-quadruplex structure of Htelo, ... previously [43]. The 32P-labeled 25-mer primer annealed to the 3¢ end of the template and could be extended by a DNA poly-merase upon the addition of the dNTPs. If completeextension of the primer...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... expression of T-bet has beenseen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse ... end of the gene, where the zebrafish and human sequences appearto be quite diverse. Together with the well-conservedgene organization, there is a conservation of syntenybetween the human and ... important in the development of a number of these cell types.They were found within the zebrafish genome, using a synteny approach in the case of t-bet and foxp3. Multiple alignments of zebrafish...

Tài liệu Báo cáo khoa học: Identiﬁcation of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... addition of the terminal galactose of the LOS [25], and Schwin-gel et al. found the lgt6 gene involved in the initialassembly of the LOS [26]. However, for lipid Abiosynthesis of the M. catarrhalis ... regarding the addition of the decanoyl and dodecanoyl acyloxyacyl residues.Our knowledge of the enzymology and moleculargenetics of lipid A biosynthesis is based mainly onstudies of the LPS ... wasperformed to study the functions of these genes and the structures of their resultant LOSs in vitro and in vivo.ResultsIdentiﬁcation of putative late acyltransferasegenes of O35ETwo putative...

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