Báo cáo khoa học: The Rab5 effector Rabaptin-5 and its isoform Rabaptin-5d differ in their ability to interact with the small GTPase Rab4 doc

... FEBS Journal 272 (2005) 37–46 ª 2004 FEBS The Rab5 effector Rabaptin-5 and its isoform Rabaptin-5d differ in their ability to interact with the small GTPase Rab4 Elena Korobko1,2, Sergey Kiselev1, ... August2004)doi:10.1111/j.1432-1033.2004.04399.x Rabaptin-5 is an effector for the small GTPase Rab5, a regulator of the early steps in endocytosis. In addition, Rabaptin-5 interacts with the small GTPase Rab4 that has been implicated in ... therefore used this assay to analyze the interaction of Rabaptin-5d with Rab4 and Rab5. Similarly to Rabaptin-5, Rabaptin-5d was not able to bind Rab4 or Rab5 in the inactive, GDP-boundform as...

Báo cáo khoa học: 2-Hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difﬁcile pdf

... 551ResultsCloning of the genes, hadI and hadBC The genes, hadI for activator or initiator and hadBCfor the two subunits of the dehydratase, were identiﬁed in the gene cluster of the putative ... experi-ments indicated that the low dehydratase ⁄ activatorratio £ 1 required to get high activity was due to the instability of the activator in the assay mixture. The activator HadI could ... for(R)-2-hydroxyisocaproyl-CoA to (E)-2-isocaprenoyl-CoA, which, however, remains to be determined. The inability to measure the hydration of the chemicallysynthesized (E)-2-isocaprenoyl-CoA could be eitherdue to the...

Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot

... regulated independently by the main fuelsources in muscle and by insulin through directlyaltering the expression of the human PDK2 and PDK4isoforms. In addition to our ﬁndings, insulin has been ... established as an insulin-sensitive target pro-tein [30]. Incubation of myoblasts with the mTOR-selective inhibitor rapamycin (100 nm) for 5 h wasemployed to further elucidate the insulin -to- PDK path-way ... Denmark). The PtdIns3Kinhibitors LY294002 and wortmannin were from AlexisCorporation (Nottingham, UK) and Sigma, respectively. The mTOR inhibitor, rapamycin, was purchased from Sig-ma and the MEK inhibitor,...

Báo cáo khoa học: "A Statistical Tree Annotator and Its Applications" pptx

... function tags. To simplify the system build-ing, we take the ﬁrst tag in training and testing and report the aggregated accuracy only in this section.#-sents #-nodes #-funcNodestraining 71,186 ... contributionsof different feature groups by incrementally addingfeatures into the system, and the results are reported in Table 5. The dummy baseline is predicting the most likely prior – the empty ... frequent labels carrying pseudotags in the development set, and tabulate their per-formance in Table 7. The 2nd column contains the number of nodes in the reference; the 3rd column the number of nodes...

Báo cáo khoa học: "Serial Combination of Rules and Statistics: A Case Study in Czech Tagging" potx

... finite-state automaton startingfrom the beginning of the sentence and passing to the right until it finds an input configuration onwhich it can operate by deletion of some parts of the input. Having performed ... ambiguitiesoccur within a paradigm, i.e. they are common to all lexemes belonging to a particular in ectionclass. For example, in Czech (as in many other in- flective languages), the nominative, the accusative and ... with them to show the differ- ence. Table 2 shows the resulting interpolationcoefficients for the tag language model using the usual linear interpolation smoothing formulawhere p( ) is the “raw”...

Báo cáo khoa học: 6-Phosphofructo-2-kinase and fructose-2,6-bisphosphatase in Trypanosomatidae Molecular characterization, database searches, modelling studies and evolutionary analysis pptx

... that in these trypanosomatid proteins, the ankyrin repeatstructures are involved in establishing interactionsbetween proteins. However, it remains to be determined with which proteins. Known interaction ... PFK-2 and FBPase-2domains in the C-terminal halves of the proteins are indicated as in Fig. 2. In the N-terminal halves, the ankyrin motifs with a primarystructure having high similarity to the ... mediate protein–protein interactions. Within a protein, the motif canform distinct units of 2–20 consecutive stacking repeats with a hydrophobic core. The units thus form scaffolds with a stability...

Báo cáo khoa học: Identiﬁcation of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis potx

... sug-gesting that the corresponding gene products werecomplementing the deﬁcient strain. In these two strainssuccinate is converted into fumarate and then oxidized to malate. The latter is further ... citrate) into pyruvate in the presence of divalent metals and in the absence ofnicotinamide cofactors [7]. Noteworthy, the activity ofMEF proteins contribute to the intracellular pHhomeostasis since ... and OADproteins contain the same conserved amino acidsfound in the active site of previously characterizedMEs, including the catalytic tyrosine and lysine resi-dues involved in the acid–base...

Báo cáo khoa học: "CLASSIFICATION OF MODALITY FUNCTION AND ITS APPLICATION ANALYSIS TO JAPANESE LANGUAGE" doc

... relations between the subordinate clause and the main clause This information is used to determine the scope of negation in the main clause. In the subordinate clause with the conjunctive particle ... Following the conjunctive analysis between the subordinate clause and the main clause, this module is activated to determine whether the modality in the main clause also operates on the subordinate ... sometimes affects the subordinate clause. Although the main clause in the following (s14) is not usually negated, the subordinate clause is. Nevertheless, the tense information in the main clause...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... thattau interacts with Fyn-SH2. Proteins harbouring SH2domains bind to phosphorylated tyrosines on their tar-get proteins, thereby coupling the activity of tyrosinekinases with intracellular ... tyrosines present on tau [17–19], and tau alsointeracts with Fyn via its Src homology (SH)3 domain[20,21]. The SH3 domain of Fyn binds to proline-richmotifs within the sequence PXXP in interacting ... that interactions between the microtu-bule-associated protein tau and the nonreceptor tyrosine kinase Fyn play acritical role in mediating synaptic toxicity and neuronal loss in response to b-amyloid...

Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx

... the sub-strate and the highly charged active site [19,20]. In contrast to the ﬁndings with Yop51 and PTP1, increas-ing the ionic strength from 100 to 500 mm enhanced the binding of InsP6 to ... beinginactivated after 10 min of treatment and 65% beinginactivated after 30 min. Incubation of the enzyme forup to an hour with 100 lm H2O2did not further inac-tivate the enzyme. Incubation ... directly with the cysteine Sc in the unoxidizedconformation. Oxidation of the catalytic cysteinedecreased the contacts made to the cysteine Scbutresulted in the formation of 17 additional interactionswith...

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