Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx

... Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation Bogachan Sahin1, Janice W. Kansy1, Angus C. Nairn2,3, ... E-mail: james.bibb@utsouthwestern.eduAbbreviations: AK, adenosine kinase; Ado, adenosine; hAK, human adenosine kinase; mAK, mouse adenosine kinase. Note: Nucleotide sequence data for the long and ... NY;7Veterans Administration Medical Center, Dallas, TX, USAThe regulation of adenosine kinase (AK) activity has thepotential to control intracellular and interstitial adenosine (Ado) concentrations....

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... functionugcgucugacaUGUACAGCcccugccaaauuuuaauaggcaatAGUAAAUAaauaacgacaagaagcaaauggAt5g24490 (1) Ribosomal protein; unknown function cucaucucuccuuacaguuuaccuguguaggaguuaggguucuugaauaaacaaugcaacaaagauuguagaagucagUGUACAUAAt4g36040 ... and 5¢-GTCAAACATTTCTCAACAACGTGTGAAGCAAACTTCTGTTGG-3¢ (reverse) to APUM-2 and 5¢-CGATGCAGAAATTCAGTAGCAACATGGTGGAACGATGTCTCA-3¢ (forward) and 5¢-GCATGAGACATCGTTCCACCATGTTGCTACTGAATTTCTGCA-3¢ (reverse) ... cucaucucuccuuacaguuuaccuguguaggaguuaggguucuugaauaaacaaugcaacaaagauuguagaagucagUGUACAUAAt4g36040 (1) Protein containing DNAJ domain;unknown functioncuacgucggacggaacugggaaaccgaucaguguugguagugaguuaacucggugaccgaguuaguagaacgaguuaauuagUGUAAAUAcgaagccaAt4g39090 (1)...

Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

... CCAACTTATGTCCCGACTCTGCATCTGTGAAAGTCaDHODH-rev, CCGGAATTCCTTATCATCAGAGCCAATTATCa-BamHI -for, GCGGATCCCGAATGTTTCGTCCAAGTATCAAATTCAAACAGTCGCak-BamHI -for, GCGGATCCCGAATGTCAAGATCAGCAATCCATGAATATGTTTTGTGCCaDHODH-rev3, CCGGAATTCTCACTTATCATCAGAGCCAATTATTTGCTCCCATGExpression ... ATGTTTCGTCCAAGTATCAAATTCZGCaURA1–3¢, TCACTTATCATCAGAGCCCa-forlong2, ATGTTTCGTCCAAGTATCAAATTCAAACAGTCGACTTTGTCCCaKDHODH-mutfor1, CACAGATGCAGAGTCGGGACATAAGTTGGGGGTTCaKDHODH-mutrev1, CCAACTTATGTCCCGACTCTGCATCTGTGAAAGTCaDHODH-rev, ... pK a2 , 9.5 ± 0.1 for CaDHODH and 9.9 ± 0.15 for DNCaDHODH.We compared the activity of CaDHODH and DNCaDHODH with a variety of native and two artiﬁ-cial electron acceptors. CaDHODH and DNCaD-HODH...

Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... endosomal ⁄ prevacuolar structures. At 10 min,punctate staining diminished and vacuolar staining in-creased, and ﬁnally punctate staining was almost lostwith predominant vacuolar staining at 30 ... Munn AL &Yang H (2005) AAA ATPases regulate membrane asso-ciation of yeast oxysterol binding proteins and sterolmetabolism. EMBO J doi:10.1038.35 Levine TP & Munro S (2001) Dual targeting ... spheroplasts and thenlysed. CPY was immunoprecipitated from the growthmedium ⁄ periplasm (extracellular) or spheroplast lysate(intracellular) using anti-CPY IgG and Protein A sepharosebeads. After...

Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus potx

... mM3,3¢-dimethoxybenzidine, and 7 Uhorseradish peroxidase. The increase in absorbance(460 nm) was compared to a standard curve, which wasprepared separately using known amounts of H2O2.Theassay was not disturbed ... a thermostatted cuvetteholder. Initially, one standard method was used for measuring NADH oxidase activity of the recombinant geneproducts. The standard assay mixture contained 100 mMpotassium ... (5¢-CGCGCCATGGCCAAGCTTTTCGAGCCAATCGAG-3¢, sense) and BG855(5¢-CGCGGGATCCCTAAACCTTCAAAGCCAGAT-3¢,antisense), with restriction sites NcoIandBamHI in bold; for noxC primer BG831 (5¢-GCGCGTCATGATGGAATGCCTTGACTTGCTGTTC-3¢,sense)andBG832(5¢-CGCGCGGATCCTCACCATTTTTCGAAGTGCGTGAG-3¢,antisense),...

Báo cáo khoa học: Molecular characterization of a novel nuclear transglutaminase that is expressed during starﬁsh embryogenesis ppt

... ACATGTACATGTATATCACTTTGAACTGGTTTTCATTAAAAAAAAAAAACCATCAATTTG 2459 AGAAGAAACAATTACTTCTTAAGTCAATTAATTTTTCTAGAAATGCAAAAGATATTCCCC 2519 TTAACAGCTGTTTGAAATGAGGCCTCGGTCTCAAGTTTAAGAGTGCCCCCATATGTAAGC ... TAAAAAGCTCCAGGAAGTTGACCCAGAAGAAATTTGTTAAGAGTTCACGGATAAGCAAGG 2639 TATTTGGATAAGGTGCATTTGTACATTTTGTGTGTACTGGTTTAGTGTAGAATTTAATTT 2699 TTTTTGGTTAATTCTGTCACAAGAACATAATTCTATGGTTACTACACAATGTTGCATCCC ... CCGCAGCTCAGTGGTGTCAAGGCCCATGTCACACTCAATGTCAAGAGTGCTTAATTTGCT 2279 P Q L S G V K A H V T L N V K S A * ATGCGAGGTCAGCATTTATCCAACCAGAAGCTTCACGGAGCTAGCTGGGCAAGGAAATTT 2339 GATAATCGCAAGAAATAATTTCCCCCCAAAAACAAAAGGTTGTTGGCTGAAAATACTTCT 2399 ACATGTACATGTATATCACTTTGAACTGGTTTTCATTAAAAAAAAAAAACCATCAATTTG...

Báo cáo khoa học: Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis docx

... Russia, eastern Asia, Austra-lia, and New Zealand, and has the potential totransmit pathogens including viruses, rickettsia and protozoan parasites that cause important human and animal diseases ... M. K. Islam1,M. A. Alim1 and Kozo Fujisaki2,31 Laboratory of Parasitic Diseases, National Institute of Animal Health, Ibaraki, Japan2 National Research Centre for Protozoan Diseases, Obihiro ... & Tipton VJ(1968) Review of Haemaphysalis (Kaiseriana) longicornisNeumann (resurrected) of Australia, New Zealand, NewCaledonia, Fiji, Japan, Korea, and Northeastern China and USSR, and...

Báo cáo khoa học: Molecular characterization of secretory proteins Rv3619c and Rv3620c fromMycobacterium tuberculosisH37Rv docx

... 190 A 2, and the buriedsurface area was 1211 A 2. The ASA for the ESAT-6–CFP-10 solution structure was 11 512 A 2, and theburied surface area was 1675 A 2. The buried surfacearea for ... Ile17, Ala21, Leu24, Ala26, Ala30, Ile31,Ile32, Val35, Leu36, Ala38, Phe41, Cys50, Phe53,Leu57, Phe61, Val63, Ile64, Ala68, Ala70, Val75,Ala77, Ala78, Met82, Val89 and Ala94 of Rv3619c and Met12, ... 353 Molecular characterization of secretory proteins Rv3619c and Rv3620c from Mycobacterium tuberculosis H37RvAnjum Mahmood1, Shubhra Srivastava1, Sarita Tripathi1, Mairaj Ahmed Ansari2,...

Báo cáo khoa học: Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference pptx

... template, the other with forwardprimer matGIHF (5¢-AACATCCTGGACAGCAAATGCAGGG-3¢) and T7-containing reverse primer (5¢-TAATACGACTCACTATAGGGAGACCGGCA TTGAGGATG CTGAT-3¢) for the antisense-strand ... Katayama H, Tominaga S, Takasuka T,Nakatsuji T, Sonobe T, Aida K & Nagasawa H (2005)Cloning and characterization of a molt-inhibitinghormone-like peptide from the prawn Marsupenaeusjaponicus. ... reactions were set up, one withT7-containing forward primer (5¢-TAATACGACTCACTATAGGGAGAAA CATC CTGG ACAGCA AATGC AGGG -3¢) and reverse primer (5¢-CCGGCATTGAGGATGCTGAT-3¢) for the sense-strand...

Báo cáo khoa học: Molecular characterization of artemin and ferritin from Artemia franciscana pot

... poly (A) tail are shaded grey. The polyadenylation signals, AATAAA, are inbold and boxed, the ATTTA sequence and its variant ATTTTA are inbold and italicized, and a G/T stretch is in bold and shaded grey.Fig. ... Molecular characterization of artemin and ferritinfromArtemia franciscanaTao Chen1,*, Reinout Amons2, James S. Clegg3, Alden H. Warner4 and Thomas H. MacRae11Department of Biology, ... obtained by a single pass from eachselected clone using a T3 primer at the 5¢ end and DNAStrider 1.2 for analysis [38].Structural characterization of artemin and ferritincDNAs and proteins Of the...

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