Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme potx
Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme potx
... malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme Abhai K. Tripathi1, Prashant V. Desai2, Anupam Pradhan1, Shabana ... by semiquantitativeanalysis of transcripts by Northern blotting and also byquantification of the enzyme protein by Western blotting.Expression of Pf MDH, Pf LDH and Pf MQO in control and drug ... containing 100 lgÆmL)1ampicillin and 25 lgÆmL)1kanamycin. A 5 mL overnightculture of transformed E. coli cells was transferred to500 mL fresh LB medium containing ampicillin and kanamycin and...
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Báo cáo khóa học: Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx
... chaperones and play arole in the unfolding of substrates and their translocationinto the cavity of the cylinder of the proteins themselves [14]. In the past decade, although ATP-dependent proteases of ... by an N-terminal domain, a centralATPase domain which includes an SSD (sensor- and sub-strate-discrimination) domain, and a C-terminal proteasedomain. The Bt-lon is a heat-inducible gene and ... deter-mined. The standard curve was plotted with the logarithm of molecular mass against Ve/V0 of the standard protein.Peptidase and ATPase assays The peptidase activity of Bt-Lon was examined...
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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
... using a box plot. The line inside each box is the median; the upper and lower limits of the box are the 75th and 25th percentiles, respectively, and the vertical bars above and below the box indicate ... and preva-lent cancers in women and a leading cause of cancer-related death [11]. As in other common cancers, the formation and progression of breast cancer is a multi-step process involving ... was created by cloning the ESR1 coding sequenceinto the EcoRI and NheI sites of pcDNA3.1 (Invitrogen). The primers used in the subcloning experiments areincluded in Table S2; all the construct...
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Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx
... nondenaturing PAGE. Posi-tions of the top and middle bands coincided well with the two bands observed with the purified enzyme. Thus, it wassuggested that the middle and top minor bands of the Ôcomponent ... showedthat the upper band consisted of the a, b and c subunits in amolar ratio of 1.0 : 1.0 : 1.2. The lower band was composed of the a and c subunits in a molar ratio of 1.0 : 0.9. When the purified enzyme ... than aD in the N-terminal and by three than bD in the C-terminal,respectively. In the final structure, all residues of the a chain(Met1–Glu555), all but N-terminal 10 residues of the bchain...
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Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot
... regulated independently by the main fuelsources in muscle and by insulin through directlyaltering the expression of the human PDK2 and PDK4isoforms. In addition to our findings, insulin has been ... returning transcript abundance of PDK2 and PDK4 to (minus fatty acids, minus insulin) values(Table 2). The ability of insulin alone to regulate PDK2 and PDK4 transcript abundance was also investigated(Fig. ... 5 h in the presence of insulin(1 lm) and U0126 (100 lm). U0126 failed to reverse the effects of insulin on PDK2 and PDK4 transcriptabundance (Fig. 2D), suggesting that the MAPKsignalling cascade...
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Báo cáo khoa học: Relaxin-3⁄ insulin-like peptide 7, a neuropeptide involved in the stress response and food intake Masaki Tanaka pptx
... minireview, the expression of relaxin-3 in the brain, and particularly its functions, including the stress response and food intake, are described.Expression of relaxin-3 in the brainRelaxin-3 ... expressed in the brain and testis. In rodent brain, ana-tomical studies have revealed its predominant expression in neurons of the nucleus incertus of the dorsal pons, and a few other regions of the ... brain-stem. On the other hand, relaxin-3-expressing nerve fibers and the relaxin-3receptors, RXFP3 and RXFP1, are widely distributed in the forebrain,with the hypothalamus being one of the...
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Tài liệu Báo cáo khoa học: "An Information-Theory-Based Feature Type Analysis for the Modelling of Statistical Parsing" docx
... types is, the headword of the currentnode (type1 ), the headword of the parent node (type2 ), the headword of the grandpa node (type3 ), the first word in the objective wordsequence (type4 ), the first ... first left brother of the parent 0.2505 1.5603 0.61455.2 The analysis to the influence of the structural relation and the structuraldistance to the predictive informationquantities of the history ... headword of the node. In the following, the units of PIQ are bits. ConclusionAmong the feature types in the same structuralposition of the parsing tree, the predictiveinformation quantity of lexical...
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Báo cáo khoa học: An acridone-producing novel multifunctional type III polyketide synthase from Huperzia serrata pot
... plant.Further structural analyses of the enzyme promise toprovide novel insights that will aid in understanding and engineering the substrate and product specificity of the CHS superfamily type III ... containing 10%glycerol and 0.2 m KCl at a flow rate of 1.0 mLÆmin)1. Enzyme reaction The standard reaction mixture contained 54 lmol of star-ter-CoA, 108 lmol of malonyl-CoA and 20 lg of the ... synthase. The catalytic triad (Cys164, His303, and Asn336), and the active site residues 197, 215, 256, 265 and 338 (numbering in M. sativa CHS) are indicated by #, and residues for the CoA binding...
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Báo cáo khoa học: An intermediate step in the evolution of ATPases ) the F1F0-ATPase from Acetobacterium woodii contains F-type and V-type rotor subunits and is capable of ATP synthesis Michael Fritz and Volker Muller ¨ docx
... motor. This is the starting pointfor a detailed analysis of the stucture and function of the rotor of the Na+F1F0-ATP synthase from A. woo-dii, the first containing V0- and F0-like ... An intermediate step in the evolution of ATPases ) the F1F0-ATPase from Acetobacterium woodii contains F -type and V -type rotor subunits and is capable of ATP synthesisMichael Fritz and ... twohairpins [20]. Most important, the ion-binding site isnot conserved in hairpin 1. If one assumes the samenumber of hairpins in V0 and F0, the rotor of V1V0-ATPases has only half the...
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Báo cáo khoa học: An alternative mechanism of product chain-length determination in type III geranylgeranyl diphosphate synthase docx
... significant role in the product determination in these mutants, probably by blocking the elongation of the prenyl chain of the products, but in an incomplete manner.We hypothesize that type III ... the enzyme activity of the S140H and S140Y mutants was decreased to 54 and 37% of that of the wild -type HPS, respectively, while the other mutants showed an activity comparable with that of the ... along the a-helix containing the G(Q/E) motif in the mutants and that residue H139 acts as the bottom of the reaction cavity in the wild -type S. cere-visiae GGPS.Fig. 3. Replacement of the amino...
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