... Effect of valine 106 on structure–function relation of cytosolic human thymidine kinase Kinetic properties and oligomerization pattern of nine substitution mutants of V106 Hanne Frederiksen*†, ... nine mutations: V106A, V106G, V106H, V106I, V106K, V106L,V106M, V106Q and V106T. We then characterized theenzymatic properties of the mutant enzymes.Theapparentnativesizesofthe–ATPformsofV106WT and ... Dimeric enzymes: V106WT, V106A, V106I and V106T; (B) tetrameric enzymes: V106G, V106H, V106K, V106L,V106M and V106Q. v is the initial velocity.Ó FEBS 2004 Effect of amino acid 106 on human TK1 (Eur....