... transcription–PCR analysis of unknown serial analysis of geneexpression tags; rSAGE, reverse serial analysis of gene expression; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis of ... PCR analysis of unknown tags on serial analysis of gene expressionWang-Jie Xu1, Qiao-Li Li1, Chen-Jiang Yao1, Zhao-Xia Wang1, Yang-Xing Zhao1 and Zhong-Dong Qiao1,21 College of Life ... compilation ª 2008 FEBSgenomic position [20], there still exists a much earlierSAGE database constructed with the use of theconventional SAGE technique, which consists of shorter tags (14 bp). Converting...