Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx

... presenilin complex (Eur. J. Biochem. 271) 385 Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and ... or from cases with presenilin 1 missense mutations, indicated no change in presenilin 1 complex mobility. Higher molecular mass presenilin 1-reactive species weredetected in brain containing presenilin ... mass complexes consisting of hetero-dimers of N- and C-terminal fragments in association with nicastrin, presenilin enhancer-2 and anterior pharynxdefective-1 proteins. Using Blue Native gel electrophoresis (BN/PAGE)...

Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-ﬁxing enzyme in the reductive tricarboxylic acid cycle ppt

... fisocitrate, and indeed, the site-specific mutagenesis of K253 to Met led to an inactive protein [23]. In addition,the alkylation of the adjacent M258 inactivated the IDH from A. vinelandii [26]. ... heresidues in Ec -IDH. Expression and purification of IDH from recombinantE. coliA high level of NADP-dependent IDH activity c ould bedetected in the cell-free extract after ind uction with IPTG.The ... regulating the d irection and flux of the RTCA cycle. For further understanding of the R TCAcycle, we are carrying out detailed investigations of eachmember of the cycle. With respect to IDH, although...

Báo cáo khoa học: Characterization of SCP-2 from Euphorbia lagascae reveals that a single Leu/Met exchange enhances sterol transfer activity pdf

... ergosterol in thestructural model of A. thaliana SCP-2. The bindingmode in (A) has higher ﬁtness than the binding mode in (B). The polar amino acids at the opening betweenhelices D and E and b-strand ... amino acids in the Triton-bound con-formation of SCP-2. The amino acids lining the cavit-ies in the E. lagascae and A. thaliana Tr-SCP-2 models and in the structure of the SCP-2-like domain of human ... understand the lipid–ligandbinding mode of plant SCP-2 and to identifyamino-acid substitutions that may explain the differ-ences in ligand-transfer activity, we used protein mod-elling and site-directed...

Báo cáo khoa học: Characterization of thermostable aminoacylase from hyperthermophilic archaeon Pyrococcus horikoshii pot

... that, in PhoACY, the mutation of one zinc ligand allows the local loosening of Zn2+ and the intervention of another Zn2+ in the metal-binding pocket, resulting in the incorrect coordination of ... Speculated zinc-binding domain of PhoACY. Zinc ion is shown by a pink sphere. The residues related to zinc binding are shown by theballs and sticks. Characterization of thermostable aminoacylase ... examined the contribution of His198 and Arg260 to the enzyme activity of PhoACY.The residues His206 in human aminoacylase-1(hACY1) and His205 in porcine aminoacylase-1(pACY1) are involved in...

Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate speciﬁcity by metal cations, and allosteric behavior of the peptidase pdf

... greater understanding of thecharacteristics of this peptidase, which would be of industrial use in the debittering of fermented foods.Results and DiscussionCloning and expression of Lc. lactis ... proteinengineering of this enzyme. In the present study, the prolidase-coding gene, pepQ,was isolated from Lactococcus lactis NRRL B-1821 and cloned. Characterization of the recombinant proteinrevealed ... ultimately produce proline-containing dipeptides,such as Xaa-Pro and Pro-Xaa. Ishibashi et al. [5]reported two interesting observations regarding pro-line-containing dipeptides: most of these dipeptidesare...

Báo cáo khoa học: Characterization of a b-N-acetylhexosaminidase and a b-N-acetylglucosaminidase/b-glucosidase from Cellulomonas ﬁmi potx

... nm using a UV trans-illuminator. Nucleotide sequences of inserts were deter-mined by primer walking and conﬁrmed by sequencing thecomplementary strand. The nucleotide sequences of hex20A and ... regions within the Streptomyces plicatusenzyme are found in the N-terminal module of unknown function and within the following regions of the catalytic module: a-helix 4 and the loops afterb-strands ... identical2.2 kb insert, which contained the 2.0 kb insert of pCF10. DNA sequencing of the inserts revealed the2.0 kb insert to be an incomplete ORF missing 20 bpat the 5¢ end and a 200 bp portion...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... dimension. A sine function wasapplied in both dimensions of the time domain beforeFourier transformation.Integration of 1D and 2D signals was performed using 1D winnmr and 2D winnmr software (Bruker, ... m2 and m3obtained from fitting with Eqn (4) ML data, as in Fig. 5, for irradiated (I) and non-irradiated (C)HeLa and MCF-7 cells. The minimum values(tm) of the parabolic fitting curve and ... phosphatidylserine5.00.0–5.0–10.05.00.0–5.0Fig. 8. Parameters m1, m2 and m3obtained from fitting with Eqn (4) ML data, as in Fig. 5. The minimum values (tm) of the para-bolic fitting curve and...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... mainly expressed during larvalmetamorphosis, in the mantle edge and the digestivegland. During the reproductive cycle, expression was high in gonads during the postspawning period butnot in ... cooperate with insulin to stimulate pro-liferation, polarization and mobility of imaginal disccells in vitro. Imaginal disc growth factors may alsoconstitute morphogenetic factors controlling embryonic and ... FEBS from the oyster Crassostrea gigas [5]. Interestingly, thisprotein, named C. gigas chitinase-like protein 1 (Cg-Clp1) was found to be involved in the control of growth and remodelling processes...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... indicate intron 6 of 75 bp in fragmentsB and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and 3¢ portion of 25 bp in intron 6 in fragments F and I. The number atthe right of each RT-PCR ... genera-tion of protein isoforms differing in amino acidsequence. Human DNASE1 pre-mRNA is transcribed from different transcription start sites, exons 1 and 1a,resulting in generation of two kinds of ... undergoesextensive alternative splicing in pancreas and QGP-1cells, resulting in the generation of normal transcripts and many alternative splicing variants initiated from both exons 1a and 1, as shown in Fig....

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... C-terminus of isoform 1 of the PM H+-ATPase of A. thaliana (AHA1) interacts with the ﬁrst 88 aminoacids of PPI1 [22], indicating that the PM H+-ATPasebinding site of PPI1 is localized therein. ... blue- lightactivation of PM H+-ATPase in guard cells of broadbeans involves protein kinase-mediated phosphoryla-tion of Ser and Thr residues in the C-terminus of thepump and 14-3-3 binding [17]. Much ... cleavage of the enzyme, indicatingthat the PPI1 binding site in the H+-ATPase C-termi-nus is localized upstream of the main tryptic cleavagesite and thus is different from the 14-3-3 bindingsite....

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