Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt

Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... molar ratio of Ca2+⁄ CaM to PhK5had been reached. The inset shows an expanded view of the indi-cated area of the spectrum. (Bottom) An overlay of spectra from the titration of Ca2+⁄ CaM ... spectrum indicative of a large conformational change upon binding of PhK5 (Fig. 4A) . These changes are observed early in the titration, at as little as 20% saturation changes arereadily apparent. Intermediate ... Ca2+-regulated control of PhK activitythrough the formation of a classical ‘compact’ CaM complex.AbbreviationsCaM, calmodulin; CaMK, CaM kinase; CaMKK, CaM kinase kinase; eNOS, endothelial...

Báo cáo khoa học: Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus inﬂuenzae pot

... (phosphocholine), 165.13 and lipid A- OH (O-deacylated lipid A) , 953.02. Relativeabundance was estimated from the area of molecular ion peak relative to the total area (expressed as percentage). Peaks representing ... H-1 and H-2 (a- conﬁguration). On the basis of the chemical shift data and the large J2,3, J3,4andTable 4. Structures of the Hep4-glycoforms of nontypeable Haemophilus inﬂuenzae (NTHi) strain ... methylation analysis, on the basis of the chemical shift data and the large J2,3, J3,4and J4,5values( 9 Hz). On the basis of low J3,4and J4,5values (<4 Hz)and chemical shift data, the...

Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt

... et al.(Eur. J. Biochem. 271) Ó FEBS 2004 Structural characterization of the human Nogo -A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal ... ¢-CGCGCGCGCGGATCCACTGGTACAAAGATTGCT-3¢(forward) a nd 5¢-CGCGCGCGCCTCGAGCTAAAATAAGTCAACTGGTTC-3¢ (reverse). A DNA fragmentencoding human Nogo-66 corresponding to residues1055–1120 of Nogo -A (Fig. 1) was ... designs of NgR antagonists.Experimental proceduresCloning and expression of the Nogo -A fragments The Nogo -A cDNA (designated KIAA 0886) was obtainedfrom the Kazusa DNA Research Institute (Kazusa-Kamatari,...

Báo cáo khoa học: Structural characterization of photosystem II complex from red alga Porphyridium cruentum retaining extrinsic subunits of the oxygen-evolving complex docx

... evolutionarily one of the most p rimitiveeukaryotic algae. The photosynthetic apparatus of red algaeappears to represent a transitional state between cyanobac-teria and photosynthetic eukaryo ... ultrastructure of red algal chloroplasts is similar to t hat of cyanobacteria.Thylakoid membranes of red algae are not differentiatedinto stacked and unstacked regions as fou nd in higher plantsand ... dispersedparticles with uniform size and shape and i s almost free of contaminants.To process the particle images by single p article a nalysis, a large data set was extracted from the images and the projections...

Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx

... Columbia, Canada The structure of the antigenic O-chain polysaccharide of Flavobacterium columnare ATCC 43622, a Gram-negativebacterium that causes columnaris disease in warm water fish,was determined ... possible candidates areLPS, capsule, fimbriae or other appendages of the bacterium, a hypothesis requiring further investigation.It is of note that the structure of the LPS O-antigen of F. columnare ... characteristic of anacetamidino group, thus identifying A as an a- FucpNAmresidue, the a- configuration being assigned from consid-eration of the anomeric proton and carbon couplingconstant data...

Báo cáo khoa học: Structural characterization of the large soluble oligomers of the GTPase effector domain of dynamin potx

... speciﬁc domains suggests that a detailed characterization of the intrinsic structural anddynamic characteristics of the individual domains has the potential to throw valuable light on the interac-tions ... dextran, molecular mass2000 kDa also appeared at the same place), and therewas also a small peak seen corresponding to the mono-mer (Fig. 1A) . This meant that the molecular mass of the major ... interac-tions of the individual domains, and the mechanismand variety of the overall functions of the full lengthprotein. As of now, the structural characteristics of only the pleckstrin homology domain...

Tài liệu Báo cáo khoa học: Structural insights into the substrate speciﬁcity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... proteasesfrom a tropical plant, Ervatamia coronariaRaka Ghosh, Sibani Chakraborty, Chandana Chakrabarti, Jiban Kanti Dattagupta andSampa BiswasCrystallography and Molecular Biology Division, Saha Institute ... studies of the cysteine protease erva-tamin A from Ervatamia coronaria. Acta Crystallogr F61, 562–564.37 Kundu S, Sundd M & Jagannadham MV (2000) Puriﬁ-cation and characterization of a stable ... S, Sundd M, Jagan-nadham MV & Dattagupta JK (1999) Crystallizationand preliminary X-ray analysis of ervatamin B and C,two thiol proteases from Ervatamia coronaria. ActaCrystallogr D 55,...

Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt

... domain X motifs are found at or near the C-termini of a2 and a3 , respectively. The a- helices a1 , a2 and a3 potentially correspond to a- helices aH, aIand aJ in the thumb of HIV-RT [13]. The domain ... single-stranded DNA substrate, whereas the panel on the right shows cleavage in a 5¢-labeled top-strand, 70 bpdouble-stranded DNA substrate. DNA cleavage activity was assayed by incubating a DNA substrate ... which has a frame-shift at the beginning of the IEP sequence; YAHH, which has a mutation affecting the active site for RT activity (RT domain 5); and D5-CGA, which has a mutationin the catalytic...

Báo cáo khoa học: Structural basis for the erythro-stereospeciﬁcity of the L-arginine oxygenase VioC in viomycin biosynthesis docx

... speciﬁc for the (S)-enantiomers [33,34]. In addition, the nonhemeoxygenase AspH from P. syringae hydroxylates freel-aspartate, l-aspartate-SNAC, and a linear nonapep-tide containing an asparagine ... presence of the two a- helical subdomains in addition to the catalytic jelly roll fold. Although the presence of these a- helical subdomains might be an evolutionary relic, the C-terminal one, at least, ... C-terminal helical subdomain. Accordingly, VioC exertsbroadened substrate speciﬁcity by accepting the analogs l-homoarginineand l-canavanine for Cb-hydroxylation.Abbreviations A- domain, adenylation...

Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary ﬁber and butyric acid pdf

... requires the application of multiple analytical approaches [8].MS has become a powerful analytical tool for the struc-tural characterization of oligosaccharides. Of severalMS techniques, the soft ... Laboratories(Mumbai, India). Wheat bran was procured from the localmarket. All other chemicals and reagents were of analyti-cal grade.Animals, induction of diabetes and dietDiabetes was induced ... kDa which are characteristic of laminin. Composition analysis of laminin was car-ried out in terms of total sugars, amino sugars and sia-lic acid (Table 2). The monosaccharide composition of oligosaccharides...

Xem thêm