... reducing it oncepurified. By using the purified recombinant protein, theconversion of reagents and products of the exopeptidase-catalyzed hydrolysis of Met1 and cyclization of Gln1 topyroglutamyl ... solvent of the matrix, and 0.5 lL of the mixturewas immediately applied to a sample probe spot.Cyclization reaction as a function of pH and temperatureIn order to determine the effect of pH and ... h.Fig. 2. Comparison of the hydrolysis reaction as a function of denaturingagent and pH. (A), (B) and (C): (d), (s )and( .) correspond toguanidinium chloride concentrations of 0 , 2 and 3M, respectively.Hydrolysis...