... following exposure to calcium iono-phore, indicating that they were correctly targeted into secretory granuleseven in the absence of serglycin. In the absence of serglycin, the fates of the serglycin-dependent ... dependent on serglycin. Together,these findings indicate that the reduced amounts of neutral proteases in the absence of serglycin is not caused by missorting into the constitutive path-way of secretion, ... were incubatedfor 120 min in the presence of 2 lm of the calcium iono- Role of serglycin in secretory granule assembly F. Henningsson et al.4910 FEBS Journal 273 (2006) 4901–4912 ª 2006 The...