Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf
Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf
... degradation of the protein. The latter half of the kinase inhibitory region and the entire extended SH2 subdomain form a single a-helix. The mapping of the true SH2 domain, and the location of its ... within SOCS3. The PEST sequence is an insertion in the SH2 domain Analysis of the secondary structure of SOCS3, and sequence alignments with SH2 domains, reveal that the PEST sequence begins ... score of > 5 (Table 1).CIS and SOCS3 have the PEST motif within the SH2 domain, while SOCS1, -5 and -7 contain PESTmotifs in the N-terminal domain. The PEST sequence in the CIS SH2 domain is...
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Báo cáo khoa học: Secondary structure of lipidated Ras bound to a lipid bilayer pptx
... mea-sured in solution. Furthermore, it resembles the mean of an ensemble of 20 structures and thus indicates the dynamics of the protein, leading to changes in second-ary structure according to the ... [19]. Therefore, the error of the secondary structure change is much lowerthan the error of the absolute secondary structure determination. The decomposition of truncated Ras in solution and of ... reflectancecrystal. The structurally sensitive amide I absorbance band in the IR wasdetected and analysed to determine the secondary structure of the pro-tein. The NMR three-dimensional structure...
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Báo cáo khoa học: Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria pptx
... and and in the 20±25 loop, as well as in the P2¢ and P 3¢positions of the binding loop. The residues with the greatestDdNHvalues are Thr16 and, more interestingly, Gln35. The majority of the ... equential, and long-range (in cluding medium-range) NOE di stance restraints per residue for SGCI (A) and SGTI (B);angular order parameters [26] of / and w torsion angles of SGCI (C) and SGTI (D) and ... key point in the mechanism of canonical serine proteinase inhibition [4]. The major target enzyme of c anonical inhibitors, e.g.trypsin or chymotrypsin, is determined primarily by the amino acid...
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Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
... production of VP2 was prepared by insertion of amplified sequences into the HindIII and BglII sites, and the plasmid for production of VP3 was pre-pared by insertion of amplified sequences into BglII and KpnI ... test interac-tions of the minor structural proteins in cells, contribu-tions of individual hydrophobic domains to theirmembrane affinity and to reveal possible functions of VP2 and VP3 in the ... proteins, created by linkingenhanced green fluorescent protein (EGFP) to their C- or N-termini, wereprepared and tested in the absence of other MPyV gene products, namely the tumor antigens and the...
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Tài liệu Báo cáo khoa học: Altered inactivation pathway of factor Va by activated protein C in the presence of heparin doc
... (1997)Anticoagulant synergism of heparin and activated protein C in vitro. Role of a novel anticoagulant mechanism of heparin,enhancement of inactivation of factor V by activated protein C.J. Clin. ... constants of FVa inactivation asUFH, but pentasaccharide did not inhibit FVa inactivation.Analysis of these data in the context of the 3D structures of APC and FVa and of simulated APC–heparin and ... coagulation cofactors, FVa and FVIIIa [8], in reactions stimulated by the APC cofactorprotein S.FVa consists of a 105 kDa heavy (A1 and A2 domains) and a 71–74-kDa light (A3, C1, and C2 domains) chainwhich...
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Báo cáo khoa học: Three-step hydroxylation of vitamin D3 by a genetically engineered CYP105A1 doc
... significance of the recombinant S. lividans cells expressing the R73V ⁄ R84A variant from the viewpoint of industrial and medical applications.ResultsExpression of the R73V⁄R84A variant of CYP105A1 ... Wat560, 587 and 593 in the binding site were included in the calculation.Cluster analysis was performed on the docked results of 100 runs using a root-mean-square tolerance of 2.0 A˚.Other parameters ... kg. After 2 and 4 weeks, the rabbit was boosted with1.0 mg of the antigen in emulsified Freund’s incompleteadjuvant, and bled 1 week after the final injection.Western blot analysis of the R73V⁄R84A...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... different plant and animal sources, with more than 400isoforms being obtained from amphibian species. Thisarticle discusses the antibacterial activity and mode of action of the N-terminal region of ... different initialsurface pressures (p0), the peptide (0.1–2 lm) was injectedinto the subphase, and the increment in surface pressure of the LPS film upon intercalation of the peptide dissolved in the ... calculated according to the equationXðFICAþ FICBÞ=n ¼XðA=MICAþ B=MICBÞ=nwhere A and B are the MICs of drug A and drug B in the combination, MICA and MICBare the MICs of drug A and drug...
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Báo cáo khoa học: Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase ppt
... isomers of cyclosarin[30] and those of p-nitrophenol analogs of sarin and soman [25]. Therefore, it was of interest to examine the stereoselectivity of cyclosarin and soman hydro-lysis exerted by ... constants(kcat⁄ Km )of1 05)106m)1Æmin)1[16]. The catalytic effi-ciency of PON1 in the hydrolysis of sarin and soman and the possibility to re-inject it in humans renderPON1 a possible candidate ... obtained for en-zymatic hydrolysis of soman with all tested PON1 vari-ants indicate the importance of the V346A mutationfor the enhancement of cyclosarin and soman hydro-lysis. The PON1 variant...
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Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt
... hydrolysis, and improving the Fig. 1. (A) The crystal structure of TaLcc1as a surface model. Domain A is presented in blue, domain B in green, and domain C in yellow. The N-glycans are shown ... Asn555. The glycan on Asn202 was in the groove between the b-bar-rels of domains A and B (Fig. 2B), and had six hydro-gen bonds, three to the main chain carbonyls at Asn6,Leu168, and Val170, and ... form the phenolic substrate-binding site of the enzyme, whereas the T2 and the T3-pair coppers form the trinuclear site that is responsible for binding and reduction of the molecular oxygen. The...
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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
... cargo binding sites, referred to as the major and minor binding sites [1]. These sites arelocated in the concave face of the protein near regions of invariant Trp and Asn arrays. The major bindingsite ... to the flexible importin-b binding domain. The importin-b binding domain is known to have an autoinhibitory function, whereby an internal NLS-likesequence competes for the importin-a binding ... site,reducing binding affinity for cargo proteins and help-ing to facilitate the release of the cargo within the nucleus [10,11]. The removal of the autoinhibitory domain to create a truncated importin-a...
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