... AGGAACAA-3¢; forward (F13S), 5¢-AGCCCCGCCAT
CACCCAGGAACAA-3¢; forward (F13Y), 5¢-
TATCCCG
CCATCACCCAGGAACAA-3¢; and reverse (F13), 3¢-CG
TAGGTGTGGGTTGTATCGG-5¢. PCR products with
blunt ... sine-bell apodization in the
< /b> 15
N dimension.
RNA transcripts and EMSA
The < /b> probe was generated as described before [9]. Labeled
RNA probe was incubated with various amounts of recom-
binant pr...
... abnormalities
a
Unexpected abnormalities
a
Abnormalities expected
by the < /b> ICU physician
Abnormalities found by
the < /b> radiologist
Unexpected abnormalities
found by the < /b> radiologist
Abnormalities ... predefined major abnormalities in 2,457 daily routine chest radiographs
Abnormalities Expected abnormalities
a
Unexpected abnormalities
a
Abnormalities expected
by the...
... 5¢-GGTATTGAGGGTCGCCATGGTTATGTTC
AATCACCA-3¢; reverse primer, 5¢-AGAGGAGAGTTAG
AGCCTTACAAGAAGGGTCCAAAGA-3¢). The < /b> PCR
product was purified, treated with T4 exonuclease to
create vector-compatible overhangs and annealed ... that LlCBP3 3A has a preference for
A
B
C
Fig. 5. Substrate preferences for LlCBP3 3A at pH 6.0. (A, B) Bind-
ing of LlCBP3 3A visualized by SDS-PAGE. (A)...
... Ohtani N, Yanagawa H, Tomita M & Itaya M (2004)
Identification of the < /b> first archaeal type 1 RNase H gene
from Halobacterium sp. NRC-1: archaeal RNase HI can
cleave an RNA-DNA junction. Biochem ... coelicolor A3 (2)
encodes a bifunctional enzyme consisting of an RNase H
domain and an acid phosphatase domain
Naoto Ohtani
1
, Natsumi Saito
1
, Masaru Tomita
1
, Mitsuhiro Itaya
1,...
... 5¢-TTCCTGGACACGCTGGT-3¢;
and antisense, 5¢-CAATCGGGGATGTCTGAG-3¢. The
< /b> b- actin primer pairs were as follows: sense, 5¢-TCGTGCGT
GACATTAAGGAG-3¢; and antisense, 5¢-ATGCCAGGGT
ACATGGTGGT-3¢. The < /b> 25 lL reaction ... of the < /b> primers
used were as follows: P1 sense, 5¢-AGTTTCGCTCTTGTCT
CCCAG-3¢; P1 antisense, 5¢-ATGGCGAAACCCTGTCTC
TAC-3¢; P2 sense, 5¢-AGACAGCCGTTTTACACGCAG-3¢;
P...
... been unambiguously established for mammalian
cells. By intercalating between membrane lipids the
< /b> two structurally unrelated membrane fluidizers that we
selected benzyl alcohol (BA) and heptanol ... (i.e. a reduction of the < /b> steady-state DPH
anisotropy value by 0.015 units) can be attained by
the < /b> administration of 30 mm BA or 4.5 mm HE. The
< /b> critical concentrati...
... data described above revealed that Lad1 acts largely,
albeit with different affinities, on the < /b> same substrates as
mammalian SDH, therefore suggesting that Lad1 may be a
fungal orthologue of this ... contribution of Xdh1 is
minor compared to that of Lad1, which is therefore of
major importance for the
< /b> L
-arabinose catabolic pathway of
H. jecorina.
Because of the < /b> poo...
... is El; the < /b>
affixed string generated by GI* that represents
its structural description is El*.
(El) abbaba
(El*) SaABbABbAaSABbAaS
Let us say that an affix
covers
elements in
an affixed ...
generates the < /b> artificial language L1 = a (b* a) *b* a.
(G1) a. S • S A b. A • B A
c. A ~ a d. B * b
e. S ) a
A noncenter-embedding affix grammar that...
... domain.
Marking synsets with field labels has a clear ad-
vantage: in general, given a polysemous word in
WordNet and a particular field label, in most of
the < /b> cases the < /b> word is disambiguated. ... it contains
all
the < /b> words of the < /b> language that
do not belong to the < /b> FL. In general the < /b> quantity
of application specific information is sma...