Báo cáo khoa học: Coiled–coil interactions modulate multimerization, mitochondrial binding and kinase activity of myotonic dystrophy protein kinase splice isoforms pptx

... Journal compilation ª 2006 FEBS Coiled–coil interactions modulate multimerization, mitochondrial binding and kinase activity of myotonic dystrophy protein kinase splice isoforms Rene´E. M. A. van ... domainmodulates myotonic dystrophy protein kinase multimerization, substrate binding, kinase activity and subcellular localization characteristics.AbbreviationsCM, coil mutant; DMPK, myotonic dystrophy ... isoforms A and C, which hasbeen described previously [5,15].Fig. 1. Myotonic dystrophy protein kinase (DMPK) isoforms and truncation mutants. Structural domain organization of DMPK splice isoforms A,...

Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in ﬁsh pdf

... initial molecu-lar activity of Sry is translated into cellular structure and organ morphology. Interestingly, Dmrt1, theancestor of Dmrt1bY, is one of the downstream effec-tors of Sry in the male ... prototype members of this group of factors are the Drosophila doublesex (dsx) and Caenor-habditis mab-3 genes. The Dmrt group of molecules ischaracterized by a conserved DNA -binding motifknown ... Consideringthat dmrt1bY has all the features of the master regula-tor of testicular differentiation in medaka (see [13] forreview) and because of the discovery of sex-chromo-some-linked dmrt1s in...

Tài liệu Báo cáo khoa học: Tet repressor mutants with altered effector binding and allostery docx

... effector of TetR known so far. The DNAfragments containing the randomized codons 14–25(C-terminal part of helix a1 and the following loop) and 93–102 (helix a6 and the b-turns N-terminal and C-terminal ... a4 and 3900 out of the helix a6 pool and obtained a total of 15 candidates with the desiredproperties. These were conﬁrmed by in vivo repression and induction determined in broth cultures of ... parts of helices a1, a4anda6(see arrows) forming the interface between the DNA -binding head and the protein core are highlighted in red in one subunit. (B) Over-lay of the induced (dark blue) and...

Tài liệu Báo cáo khoa học: "Japanese Dependency Parsing Using Co-occurrence Information and a Combination of Case Elements" pdf

... possible modifyees of “keisatsu-de” are “arui-teiru” and “hogo-shita”. Taking into account in-formation about the co-occurrence of “keisatsu-de” and “arui-teiru” and of “keisatsu-de” and “hogo-shita” ... probability of the case element set (bunsetsu set) and the co-occurrence probability of the particle set, and weselect theˆT that maximizes the probability.Some of the co-occurrence probabilities of ... balance of the two proba-bilities, and is ﬁxed to the best value by consider-ing development data (different from the trainingdata)1.RerankingCandidate 1Candidate 2Candidate 3Candidate...

Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf

... is due toCatD, and the decrease in activity can be assigned to CatE. The activity of CatE and CatD in cytosolic, endosomal and lysosomal fractions of B cells,dendritic cells and human keratinocytes ... on CatD activity (Fig. 4B). This approach fordepleting proteinase activity from complex biologicalsamples is ﬂexible and can be used for other proteinasesas well. Activity of Cat E and CatD ... activity CaD activity TAPACatE activity CaD activity ABCFig. 5. Distribution of TAPA, CatE and CatD activity in subcellularfractions of the cell lines (A) HaCaT, (B) WT100 and (C) DCs. Equalamounts...

Báo cáo khóa học: Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells potx

... relevant to this binding. Some of theseFig. 10. Prediction of nucleolin -binding sites in human lactoferrin (hLf).Sequences of the N2 and C2 domains of hLf, bovine lactoferrin (bLf) and human transferrin ... Properties of the iron -binding site of the N-terminal lobe of human and bovine lactotransferrins. Importance of the glycanmoiety and of the non-covalent interactions between the N- and C-terminal ... Characterization of the anti-HIV eﬀects of native lactoferrin and other milk proteins and protein- derivedpeptides. Antiviral Res. 55, 341–355.5. Moriuchi, M. & Moriuchi, H. (2001) A milk protein lactoferrinenhances...

Báo cáo khoa học: Insulin like growth factor-1-induced phosphorylation and altered distribution of tuberous sclerosis complex (TSC)1⁄TSC2 in C2C12 myotubes pptx

... Distributions of TSC1 and TSC2 proteins in the membrane fraction and the cytosolic fraction. Alterations in therelative amounts of both TSC1 and TSC2 proteins were quantiﬁed as a percentage of control ... asmall G protein. The GTP-bound active form of Rhebstrongly stimulates mTOR and its downstream targets,such as the ribosomal S6 kinase 1 (S6K1) and theeukaryotic initiation factor 4E -binding protein ... TSC1 and TSC2 proteins were detected in both membrane and cytosolic fractions (Fig. 2A). After 1 h of stimulationwith IGF-1, there was an increase in the relativeamounts of both TSC1 and TSC2 proteins...

Báo cáo khoa học: "A GENERATIVE GRAMMAR APPROACH FOR THE MORPHOLOGIC AND MORPHOSYNTACTIC ANALYSIS OF ITALIAN" ppt

... classes of endings: andare (to go), for example, admits two different stems, vad (go) and and (went); so there exist two subclasses of endings named respectively dvl andare and dr2 andare. ... prep.simple 2 andare vad dv 1 _andare v.intran.simple 1 andare and I dv2. andar© v.intran.simple I The other sets of data are contained in the Prolog workspace and are structured as tables of a relational ... the table and the syntactic category of pronoun. Other two sets of data have been defined in order to handle fixed sequences of words, such as proper names and idioms. The set of the most...

Báo cáo khoa học: A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase pptx

... of reduction of the FMN cofactor was very small,amounting to 0.6% and 3% of the wild-type rate forthe D137L and K109L ⁄ D137L protein variants,respectively. The rate of reduction for the other two protein ... with binding of NADPH and ⁄ or delivery of a hydride tothe ﬂavin cofacor and hence K109 plays a dual role bysupporting tetramer assembly through its interactionwith D137 and stabilization of ... Institute of Biochemistry, Graz University of Technology, Austria2 Institute of Molecular Biosciences, University of Graz, Austria3 Institute of Biochemistry, University of Zu¨rich, Switzerland4...

Báo cáo khoa học: Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis ppt

... theexposure of hydrophobic regions during the urea-induced unfolding of W243 mutants compared withthe wild-type DAAO.Kinetics of reconstitution of apoprotein with FADBecause of the weaker binding of ... FAD cofactor tothe apoprotein of the W243 mutants, we studied thekinetics of reconstitution of the apoprotein–FAD com-plexes under pseudo-ﬁrst-order conditions (10- and 20-fold excess of FAD) ... by theabsence of major changes in catalytic activity, and altered the protein in a different way. Some of theproperties of the W243Y mutant are similar to those of the wild-type protein (e.g....

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