Báo cáo khoa học: Structural insights into mechanisms of non-nucleoside drug resistance for HIV-1 reverse transcriptases mutated at codons 101 or 138 pot

... 2006 The Authors Journal compilation ª 2006 FEBS Structural insights into mechanisms of non-nucleoside drug resistance for HIV-1 reverse transcriptases mutated at codons 101 or 138 Jingshan Ren1, ... priority for continued efforts to treat HIV.Experimental proceduresCrystallization and data collectionExpression vectors for HIV-1 RT (HXB-2 isolate) contain-ing either Lys101Glu or Glu138Lys ... 1dtt (for PETT-2) [31].Data depositionCoordinates and structure factors for all of the HIV-1 RTmutant structures reported here have been deposited in theProtein Data Bank for immediate release...

Tài liệu Báo cáo khoa học: Structural insights into the substrate speciﬁcity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... compriseddenaturation at 95 °C for 60 s, annealing at 60 °C for 90 s,and extension at 72 °C for 90 s) and a final extension step at 72 °C for 15 min. The forward primers for PCR weredesigned according ... predena-turation step at 95 °C for 5 min, 35 cycles of two-stepamplifications (the first five cycles comprised denaturation at 95 °C for 60 s, annealing at 50 °C for 90 s, and exten-sion at 72 °C for ... Electron Cor-poration, Rockville, MD, USA). The range of substrateconcentrations and the time of incubation were standardized for each substrate. An extinction coefficient of 8800 at 410 nm for pNA...

Báo cáo khoa học: New insights into evolution of crustacean hyperglycaemic hormone in decapods – ﬁrst characterization in Anomura pdf

... GasCHH-U1 for 3¢ RACE and Pab ⁄ GasCHH-D1 for 5¢ RACE under the following conditions: five cycles of 94 °C for 30 s and 72 °C for 2 min, five cycles of 94 °C for 30 s, 70 °C for 30 s and 72 °C for 2 ... T)TGCCAACCATCTG-3¢ (antisense), corresponding toQMVG(K ⁄ R)K(70–75). After a denaturation step at 94 °C for 3 min, 35 amplification cycles were carried out(94 °C for 30 s, 58 °C for 30 s, 72 ° C for ... min,25 cycles of 94 °C for 30 s, 68 °C for 30 s and 72 °C for 2 min, and 72 °C for 10 min as a final extension step.Nested PCR was performed realized on 1 ⁄ 150th of the volume of the first PCRs,...

Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

... amounts of core protein 1 and coreprotein 2 were relatively unaffected. Therefore, the absence of subunit 6 appeared to alter the rates of processing of two of the redox subunits and caused minor ... assembly of the yeast bc1complex from thelaboratories of Berden [30] and Tzagoloff [31]. It has beendemonstrated previously that gene expression, import of proteins into mitochondria and sorting ... in anincrease in the ratio of intermediate to mature cyto-chrome c1and a disappearance of the intermediate form of the Rieske protein. At the same time, the levels of subunits 7, 8 and 9...

Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx

... indicated in green for the cofactor ThDP, in blue for the activator ADP, and in orange for the substrate (here PADP). Differ-ent amino acid residues at the substrate binding site are indicated ... A˚ for 1023 super-imposed Ca atom pairs of 2q27 and 2q29, and rmsd0.16 A˚ for 993 superimposed Ca atom pairs of 2q28and 2q29), indicating that binding of the activatorADP or the inhibitor ... [3,4] for OfODC. Thetyrosine residues 118 and 478 (the latter in an uncom-mon side-chain conformation) stabilize the oxalyl moi-ety of the substrate as demonstrated for thecorresponding OfODC...

Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot

... respectively. For clarity of presentation, only the most abundant linkage and /or sequence informa-tive ions are schematically illustrated and annotated. The nomenclature for the ion series follows that ... and Mr Sz-Wei Wu for the MALDI-TOF/TOFMS/MS analyses of sugars. Mass spectrometrydata were acquired at the NRPGM Core Facilities for Proteomics, Academia Sinica, supported by aNational Science ... analyses, operated in a precursor ion discov-ery mode to optimize for glycopeptide detection. For the heavy chain, four distinct sets of glycopeptideswere detected, corresponding to glycoforms of trypticpeptides...

Báo cáo khoa học: New insights into the P-glycoprotein-mediated efﬂuxes of rhodamines doc

... whatever the type of cells i.e. either drug- sensitive or drug- resistant, at the steady-state (s) the rate of rhodamine influx (V+)sis equal to that of rhodamine efflux;(c) for drug- resistant ... V+,therateofpassiveuptakeforrhodamine, is equal to the number of moles that enter bypassive diffusion into one cell per second; V–,therateofpassive efflux for rhodamine, is equal to the number of moles ... uncontrolled, use of thesedrugs has led to the emergence of defence mechanisms that, at present, are the major drawback of the drug- basedtreatment of infectious diseases and cancers. Such resistance is...

Báo cáo khoa học: Recent insights into cerebral cavernous malformations: the molecular genetics of CCM pot

... molecular genetics data haveprovided useful information for clinical care of thepatients and were an important step towards theunderstanding of the mechanisms of this disorder. Thisminireview ... retinoblastoma or othervascular malformations [29,30]. According to thishypothesis, CCM formation would be caused by acomplete loss, within affected cells, of the two alleles of a given CCM gene. Loss of ... affected relative and therefore present assporadic cases. Combined use of clinical and MRIKeywordsangiogenesis; CCM1; CCM2; CCM3;cerebral cavernous malformations; cerebralhemorrhage; KRIT1;...

Báo cáo khoa học: Recent insights into cerebral cavernous malformations: animal models of CCM and the human phenotype pptx

... of the underlying biology of lesion formation. In thisregard, cerebral cavernous malformation as a geneticdisorder offers unique opportunities to understandthe biology of vascular malformations. ... blood–brain barrier of a CCM [19] or if inﬂammatory actionis part of the mechanism of pathogenesis leading toformation of aberrant blood vessels. It is intriguingthat the mitogen-activated protein ... speciﬁcity of CCMgene function, another important question of diseasepathogenesis is that of a triggering event – what eventson a molecular, cellular or physiological level lead tothe formation of...

Báo cáo khoa học: Recent insights into cerebral cavernous malformations: a complex jigsaw puzzle under construction doc

... Rudnik-Schoneborn S, Bertalanffy H, Sure U & Felbor U(2008) Novel CCM1, CCM2, and CCM3 mutations inpatients with cerebral cavernous malformations: in-frame deletion in CCM2 prevents formation of ... (2006) An inven-tory of mucin genes in the chicken genome shows thatthe mucin domain of Muc13 is encoded by multipleexons and that ovomucin is part of a locus of relatedgel-forming mucins. BMC ... analyses of human and zebraﬁsh 18-amino acid in-frame deletionpave the way for domain mapping of the cerebralcavernous malformation 3 protein. Hum Mutat 30,1003 1011 .55 Preisinger C, Short B,...

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