Báo cáo khoa học: Molecular mass of macromolecules and subunits and the quaternary structure of hemoglobin from the microcrustacean Daphnia magna ppt

... approach:(a) the determination of the molecular mass of the native Hb complex and its subunits as well as the number of subunits in the macromolecule; (b) evidencefor glycosylation of their pre-A ... al. Structure of Daphnia magna hemoglobin FEBS Journal 273 (2006) 3393–3410 ª 2006 The Authors Journal compilation ª 2006 FEBS 3399 Molecular mass of Hb subunits The reported molecular mass of Daphnia ... annelid and vestimentiferanhemoglobins of about 3600 kDa, which are composed of globin and nonglobin subunits [1].Speciﬁcally, the structure and function of the hemo-globin (Hb) of the microcrustacean...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... some of the key ﬁndings that have led to our current under-standing of the molecular mode of action of glypho-sate and the molecular basis for glyphosate resistance. Structure and function of ... (blue) structures shows the dif-ferent conformations of the main chain of the a2–a3 loop, seearrows [49]. For the sake of clarity, only the FAD and the ligandbelonging to the wild-type GO structure ... the nature of the active site or the mode of action of glyphosate. A dec-ade later, the crystal structure of EPSPS was deter-mined in complex with S3P and glyphosate [15]. The compactness of...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... Cloning of the BmIVD gene. (A) RT-PCR analysis revealed the BmIVD bandampliﬁed from whole-body RNA of standardstrains p50T and c108T as well as the skumutant. Migrations of the molecular mass marker ... combination of or and sku phenotypes. The lowerpart indicates the representative results of genomic DNA directsequencing of PCR products harbouring the SNP 1127G>T of BmIVD from each of the three ... respectively, of the neighbouring monomervia a hydrogen bond. These results suggest that the G376V mutation alters the structure of the loopregion and affects the interactions between monomers and with...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identiﬁed in each step of the screen. (C) Distribution of the 63 distinct sequences in relation of their position in the ... that of other well-characterized members of PUF family. These proteinsmust bind to transcripts in the plant and regulate theirtranslation and ⁄ or turnover.A large number of APUM target candidates: ... Double-strandedcDNA was digested with AluI, HaeIII, DraI and SspI and then fractionated on a 2% agarose gel. Fragments ranging from 50 to 150 bp were puriﬁed from the gel and ligated to the pRH5¢...

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

... organelle and membrane proteins are synthe-sized and folded with the assistance of molecular chap-erones and other folding factors in the endoplasmicreticulum (ER). In many cases, the process of ... Identiﬁcation of the initiation codonsin GmPDIL-3a and Gm-PDIL-3b mRNAs. (A)Schematic representation of the structure of GmPDIL-3a and GmPDIL-3b mRNAs. The putative ORFs (gray boxes) of GmPDIL-3a and ... of the 53 kDa spot (5.3) was identical to a pI value calcu-lated from the amino acid sequence of GmPDIL-3a, and the isoelectric point of the 57 kDa spot (5.1) wasconsistent with that from the...

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... terminal in the sinus gland, as a result of late and progressiveisomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonaltract [39,41]. The immunohistochemical ... microscopystudy of the neuronal endings in the sinus gland todetermine the subcellular localization of the differentepimers. Taken together, these results allow a discus-sion of the existence of enzyme(s) ... elucidated. The demonstration of the presence of a d-Phe3 residue in CHH A and B from the H. americanus some years ago [21] opened the possibil-ity that a d-residue may be present in one of the VIHisoforms...

Tài liệu Báo cáo khoa học: Molecular determinants of ligand speciﬁcity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc

... beobtained on the pocket site of CtCBM11 and, in par-ticular, the identiﬁcation of the atoms of the ligandthat are closer to the protein when the complexis formed. The ligands cellobiose, ... aH1a and bH1a, the anomeric protons of the reducing end of the oligosaccharide.In the region between 3.63 and 3.52 p.p.m., despite of the presence of STD signals, the individual contri-butions of ... clearthat there is a common binding site at the Ct CBM11ABCFig. 4. Representation of the conformations of the 3D structure of binding of the differ-ent ligands obtained by docking. (A) a- (red)and...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... affects the small joints of the hands and feet. The inflammatory process is characterized by infiltration of inflammatory cellsinto the joints, leading to proliferation of synoviocytes and destruction ... source of MCP-1 ⁄CCL2 production [19]. The levels of MCP-1 ⁄ CCL2correlate significantly with the levels of IL-1b, IL-6 and IL-8 ⁄ CXCL8 in culture supernatants of synovium from RA patients, and the ... protective against the development of RA [61]. The gene copy number variations of CCL3-like 1(CCL3L1), a nonallelic isoform of CCL3 encoded bydifferent genes and a potent ligand for CCR1 and CCR5, influenced...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: role of environmental factors pdf

... 0.5–1% of the popu-lation and causes progressive joint destruction thatleads to the restriction of activities of daily living and deterioration of quality of life. Although the patho-genesis of ... 0.5–1% of the population. RA causes progressive joint destructionthat leads to the restriction of activities of daily living and deterioration of quality of life. Although the pathogenesis of RA ... impli-cated in the control of infection and that is expressedon the surface of lymphoblastoid cell lines and on the envelope of the budding virion during EBV replication.Table 1. Risk and defensive...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: role of transcription factors ppt

... Considering the wide array of events under the control of NF-jB, including cytokine and cyclo-oxygenase-2 expression, osteoclast differentiation and apoptosis, and the impact of these events on the ... tested the effect of PPAR-a and PPAR-cligands in vivo on the progression and severity of adju-vant-induced arthritis in female Lewis rats and foundthat pioglitazone and fenofibrate suppressed the ... programmed cell death and has an impor-tant role in the development and homeostasis of the immune, hepatic and nervous systems. The embryoniclethality of RelA-deficient mice was one of the firstindications...

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