Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot
Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot
... 3369 Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase Sandrine ... and the GAPDH substrate, BPGA.To date, mapping of the interaction site of CP12 had not studied but our results give some clues with regard to the binding of CP12 to GAPDH. The impact of CP12 ... vitro the GAPDH ⁄ CP12 complex.Involvement of the negative charge of the residue D36 of CP12 in the interaction between CP12 and GAPDHIn order to confirm that residues from the central part of CP12...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... His145Asnmutations on the affinity of metal bindingto arginase IITo further examine the effects of the His120fiAsnand His145fiAsn mutations on the interaction of the enzyme with manganese ions, ... addition to removal of the more weakly bound Mn2+, the lower affinity for thatmore tightly bound to the protein may explain the absence of Mn2+ from the EDTA-treated species of the His145Asn variant. ... lgÆmL)1, for the wild-type, His120Asn, His145Asn and Asn149Asp variants,respectively. The line in (B) is for the average of experimentalvalues for all the enzyme variants. Interaction of arginase...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... Trp425 of Dex49A, which is the corresponding residue of Trp402 of IPU, is reportedly located in the vicinity of the active site and could form a binding site for a glucosyl unit i n subsite)1 ... (Fig. 4B). Together with the observations from the site- directed mutagenesis, Trp402 of IPU appears to be aresidue participating in subsite )1. The r esidues that form potential subsites )1and)2are ... was added every24 h for the maintenance of 0.5% (v/v) methanol as the carbon source and i nducer. The expressi on of IPU mutantswas performed using the same procedure as for the wild-type IPU.SubstratesPullulan...
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Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc
... between ,, and the other measures, with the exception of mere co-occurrence frequency.Summing up, t-test – with a few exceptionsaround the first 5% of the data in the SLs –leads to the overall ... provides a base-line for the interpretation of the precision curves.General findings from the precision graphs are:(i) It is only useful to consider the first halves of the SLs, as the measures approximate ... assess the performance of AMs withindifferent frequency strata. For instance, there isa widely held belief thatand are inferiorto other measures because they overestimate the collocativity of...
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Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
... decay (for most of them obtained with tryptophan) and theirmolecular masses (Fig. 10). We compared these data with the results of calculations of their hydrodynamic propertiesperformed from their ... direct exploitation of the structural results of the former to explain the mechanism of CaM activation of the latter. Crystallizationand resolution of the 3D structures of AC and AC–CaM,which ... however,that the activation by CaM is the result of a largeconformational change involving a major portion of the protein [23].In the absence of 3D data describing the interaction of CaM with AC,...
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Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx
... diminish the immunoblot signals for bacitracin at the site of PDI (Fig. 4C). These data support the view of a robustcovalent PDI–bacitracin interaction. We hypothesized that the thiazoline ring of ... ion mode CID-TOF ⁄ TOF MS analysis of the same precursor yielded the corresponding signaturepeaks (m ⁄ z 1404.88, 1438.82 and 1470.80) of the otherpart of the disulfide bond, i.e. the negatively ... and the thiol form of bacitracin A. The thiol form of the thiazoline ring of bacitracin A and Cys345are shown as chemical structures, the rest of bacitracin A as ‘Bacitracin A’, and all other...
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Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
... consisting of VHand VLregions with ⁄ without the VHleadersequence at the N-terminal and with ⁄ without the CL(j) region behind the VLregion. None of the con-structs contained the KDEL ... antigen. Therefore, large-scalelibraries and extensive screening are required for the selection of the antigen-specific antibody fragments. On the other hand, the antibody fragments isolated from hybridomas ... These results indicate that the scFv genes with signal sequence are translated in the ER, and,after cleavage of the signal peptide sequence, are trans-located from the ER into the cytosol without...
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Báo cáo khoa học: Principles behind the multifarious control of signal transduction ERK phosphorylation and kinase/phosphatase control potx
... how the second kinase in the cascade determines the time dependence of the activity of the third kinase, we varied the Vmax of the secondkinase reaction and recalculated the concentration of the ... are controlled by the kinases or phosphatasesin the system, and whether they are controlled differ-entially by some or all of them. On the basis of the antagonistic action of the kinases and ... as the total area under the Table 1. Control of any out of four characteristics of the time dependent signal by any of the molecular processes in the cascade. The signal is taken to reside in the...
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Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx
... the N domain of TnC bindsin a calcium-dependent manner to the C domain of TnI, releasing the inhibitory effect of the latter on the actomyosin interaction. While the crys-tal structure of the ... to the larger chimera. These resultsare similar with the results observed for the binarycomplexes formed by TnC(1–91) and TnI(1–147), andTnC and TnI [16] and agree with the hypothesis that the ... the interaction with region114–137 of TnI could facilitate the dissociation of region 166–182 of TnI from the thin filament.Final comments With the results presented in this work, combined with others...
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Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf
... ompatible with the result on t he interactions of soluble fusion proteinfragments of Semliki Forest virus with membrane [10]. Thus the i nsensitivity of the normalized intensity to variation in the ... rate of 50 nm Æmin)1 with a time constant of 2 s, step resolution of 0.1 nm, and bandwidth of 1 nm. The final spectra weretaken from the average of five s cans. The VARSELECprogram was used for ... rise of the l atter plot (from x ¼ 1) reflects that the self-association of the peptide is not tight ( on the scale of self-quenchingdistance of rhodamine, 15 A˚[17]). The deviation of the observed...
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Từ khóa: proceedings of the american society of civil engineers journal of the hydraulics divisionbáo cáo khoa họcbáo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)Chiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam