... replicatedthree times.Fatty acid analysisMicroalgae or yeast cells were harvested by centrifugation.Total fatty acids were extracted and transmethylated aspreviously described [14]. Fatty acid methyl esters ... dihydroxylated substrates.AbbreviationsARA, arachidonic acid; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; EST, expressed sequence tag; FAME, fatty acid methylester; gDNA, genomic DNA; LCB, long ... two novel microalgalenzymes involved in the conversion of the x3-fatty acid, eicosapentaenoic acid, into docosahexaenoic acid. Bio-chem J 384, 357–366.16 Meyer A, Kirsch H, Domergue F, Abbadi...