... pairing [33,34], onemay reasonably assume there should not be an obviousFig. 1. Amino acid sequences and in vitro refolding pathway of PIP and HPI. (A) Amino acid sequences of PIP and HPI. Amino ... Kuwajima, K., Ikeguchi, M., Sugawara, T., Hiraoka, Y. & Sugai,S. (1990) Kinetics of disulfide bond reduction in alpha-lactalbuminby dithiothreitol and molecular basis of superreactivity of ... pure(Amersham Biosciences, Piscataway, NJ). Ultra puredithiothreitol was from Sigma. Ultra pure urea and guanidine-HCl were from Promega. Acetonitrile and tri-fluoroacetic acid were of HPLC grade....