... Coaddition of the twoantibodies resulted in the loss of bands C1, C2, and C3 (lane13). In contrast, the use of a control monoclonal antibodyagainst SREBP did not affect the formation of any of thecomplexes ... mL of 25 mMTris/HCl buffer, pH 7.4, 5 mMKCl, 0.7 mMCaCl2,137mMNaCl, 0.6 mMNa2HPO4, 0.5 mMMgCl2,containing 5 lgoftestplasmidDNA,5lg of phRL-TKDNA (Promega), and 50 lgÆmL)1 of ... 150 ng of expressionvector (pPacSp1 or pPacUSp3) was mixed with 50 ng of pDNaseII()249/+72)-Luc, and the total amount of DNAadjusted to 200 ng with pPac0. The DNA was mixed with0.6 lLofFuGENE6in100lL...